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验证数据展示

  • WB analysis using 83809-1-RR (same clone as 83809-1-PBS)

    WB analysis using 83809-1-RR (same clone as 83809-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83809-1-RR (TP53BP1-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83809-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 83809-1-RR (same clone as 83809-1-PBS)

    IHC staining of human ovary cancer using 83809-1-RR (same clone as 83809-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83809-1-RR (TP53BP1-Specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83809-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83809-1-RR (same clone as 83809-1-PBS)

    IF Staining of HeLa using 83809-1-RR (same clone as 83809-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TP53BP1-Specific antibody (83809-1-RR, Clone: 240638C6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83809-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83809-1-RR (same clone as 83809-1-PBS)

    FC experiment of HeLa using 83809-1-RR (same clone as 83809-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug TP53BP1-Specific Recombinant antibody (83809-1-RR, Clone:240638C6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83809-1-PBS in a different storage buffer formulation.

TP53BP1-Specific Recombinant monoclonal antibody, PBS Only

TP53BP1-Specific Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83809-1-PBS

产品说明书

CloneNo. 240638C6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

TP53BP1, p53-binding protein 1, p53 binding protein 1, p202, FLJ41424

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83809-1-PBS targets TP53BP1-Specific in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tumor protein p53 binding protein 1
别名 TP53BP1, p53-binding protein 1, p53 binding protein 1, p202, FLJ41424
计算分子量 214 kDa
观测分子量450 kDa
GenBank蛋白编号NM_005657
基因名称 TP53BP1
Gene ID (NCBI) 7158
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ12888
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TP53BP1, also named as p53BP1, p202, 53BP1 and FLJ41424, may have a role in checkpoint signaling during mitosis. TP53BP1 enhances TP53-mediated transcriptional activation. TP53BP1 plays a role in the response to DNA damage.

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