验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, MOLT-4 cells, Raji cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
34301-1-AP targets TOX in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag41257 Product name: Recombinant human TOX protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 50-235 aa of BC016665 Sequence: DYVPASQSYPGPSLESEDFNIPPITPPSLPDHSLVHLNEVESGYHSLCHPMNHNGLLPFHPQNMDLPEITVSNMLGQDGTLLSNSISVMPDIRNPEGTQYSSHPQMAAMRPRGQPADIRQQPGMMPHGQLTTINQSQLSAQLGLNMGGSNVPHNSPSPPGSKSATPSPSSSVHEDEGDDTSKINGG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | thymocyte selection-associated high mobility group box |
| 别名 | KIAA0808, Thymocyte selection-associated high mobility group box protein TOX, TOX1 |
| 计算分子量 | 526 aa, 58 kDa |
| 观测分子量 | 57~70 kDa |
| GenBank蛋白编号 | BC016665 |
| 基因名称 | TOX |
| Gene ID (NCBI) | 9760 |
| RRID | AB_3743187 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | O94900 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Thymocyte selection-associated high-mobility group box (TOX) is a DNA-binding factor that can regulate transcription by modifying local chromatin structure and modulating the formation of multi-protein complexes. TOX has multiple roles in the development of the adaptive immune system including development of CD4 T cells, NK cells and lymph node organogenesis (PMID: 35485425). Two bands detected at ~63 kDa and ~57kDa were presumed to be the unmodified form and the post-translationally form of TOX, respectively,(PMID: 32106280).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TOX antibody 34301-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
