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验证数据展示

  • WB analysis using RMX00047

    WB analysis using RMX00047

    Various lysates were subjected to SDS PAGE followed by western blot with RMX00047 (TORC2 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using RMX00047

    IHC staining of human skin cancer using RMX00047

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using RMX00047 (CRTC2,TORC2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CRTC2 antibody included
  • IF Staining of HepG2 using RMX00047

    IF Staining of HepG2 using RMX00047

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CRTC2,TORC2 antibody (RMX00047) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Multi-rAb® TORC2 Multi-Recombinant antibody

TORC2 Multi-Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. RMX00047

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

CRTC2, CRTC2,TORC2, CREB-regulated transcription coactivator 2, TORC 2, Transducer of CREB protein 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA2780 cells, HEK-293 cells, HepG2 cells, Ramos cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CRTC2 Ready-To-Use IHC Kit. CRTC2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

RMX00047 targets TORC2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

Recombinant protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Multi-Recombinant
产品类型 Antibody
全称 CREB regulated transcription coactivator 2
别名 CRTC2, CRTC2,TORC2, CREB-regulated transcription coactivator 2, TORC 2, Transducer of CREB protein 2
计算分子量 693 aa, 73 kDa
观测分子量80 kDa
GenBank蛋白编号BC053562
基因名称 TORC2
Gene ID (NCBI) 200186
RRIDAB_3750099
偶联类型 Unconjugated
形式Liquid
纯化方式N/A
UNIPROT IDQ53ET0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CRTC2, also named as TORC2, belongs to the TORC family. It is a transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. It acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. CRTC2 enhances the interaction of CREB1 with TAF4. Ir regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. CRTC2 regulates the expression of specific genes such as the steroidogenic gene, StAR. TORC2 was recently shown to be an important regulator of gluconeogenesis in the livers of mammals. It is one of the other key regulators of CRE-dependent MIE gene expression in NT2 cells. This regulation is linked to VIP-induced TORC2 dephosphorylation and translocation to the nucleus. (PMID: 19369332, 20504934 ) .

实验方案

Product Specific Protocols
IF protocol for TORC2 antibody RMX00047Download protocol
IHC protocol for TORC2 antibody RMX00047Download protocol
WB protocol for TORC2 antibody RMX00047Download protocol
Standard Protocols
Click here to view our Standard Protocols
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