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验证数据展示

  • WB analysis of HEK-293 using 10647-1-AP

    WB analysis of HEK-293 using 10647-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10647-1-AP (TOR3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 10647-1-AP

    WB analysis using 10647-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10647-1-AP (TOR3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 10647-1-AP

    WB analysis of HEK-293 using 10647-1-AP

    WB result of TOR3A antibody (10647-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOR3A transfected HEK-293 cells.

  • IF Staining of HEK-293 using 10647-1-AP

    IF Staining of HEK-293 using 10647-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 10647-1-AP (TOR3A antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

TOR3A Polyclonal antibody

TOR3A Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 10647-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

torsin family 3, member A, Torsin family 3 member A, Torsin 3A, ATP-dependent interferon-responsive protein, ADIR2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, Raji cells, PC-3 cells
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:300-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10647-1-AP targets TOR3A in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag1012

Product name: Recombinant human TOR3A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 97-397 aa of BC007571

Sequence: YLDLLTTWYCSFKDCCPRGDCRISNNFTGLEWDLNVRLHGQHLVQQLVLRTVRGYLETPQPEKALALSFHGWSGTGKNFVARMLVENLYRDGLMSDCVRMFIATFHFPHPKYVDLYKEQLMSQIRETQQLCHQTLFIFDEAEKLHPGLLEVLGPHLERRAPEGHRAESPWTIFLFLSNLRGDIINEVVLKLLKAGWSREEITMEHLEPHLQAEIVETIDNGFGHSRLVKENLIDYFIPFLPLEYRHVRLCARDAFLSQELLYKEETLDEIAQMMVYVPKEEQLFSSQGCKSISQRINYFLS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 torsin family 3, member A
别名 torsin family 3, member A, Torsin family 3 member A, Torsin 3A, ATP-dependent interferon-responsive protein, ADIR2
计算分子量 46 kDa
观测分子量 39-42 kDa, 46-52 kDa
GenBank蛋白编号BC007571
基因名称 TOR3A
Gene ID (NCBI) 64222
RRIDAB_2303598
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H497
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TOR3A antibody 10647-1-APDownload protocol
IF protocol for TOR3A antibody 10647-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols