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验证数据展示

  • WB analysis of mouse brain using 19511-1-AP

    WB analysis of mouse brain using 19511-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 19511-1-AP (TOR2A antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 19511-1-AP

    IF Staining of HepG2 using 19511-1-AP

    Immunofluorescent analysis of HepG2 cells using 19511-1-AP (TOR2A antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

  • IHC staining of human stomach cancer using 19511-1-AP

    IHC staining of human stomach cancer using 19511-1-AP

    Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 19511-1-AP (TOR2A antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TOR2A Polyclonal antibody

TOR2A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 19511-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

TORP1, Salusin-beta, Salusin-alpha, PSEC0218, Prosalusin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, mouse liver tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

19511-1-AP targets TOR2A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 torsin family 2, member A
别名 TORP1, Salusin-beta, Salusin-alpha, PSEC0218, Prosalusin
计算分子量 36 kDa
观测分子量 40-45 kDa
GenBank蛋白编号NM_001085347
基因名称 TOR2A
Gene ID (NCBI) 27433
RRIDAB_10642949
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N2E6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TOR2A, also named as TORP1, UNQ6408/PRO21181, HEMBA1005096 and PSEC0218, belongs to the clpA/clpB family and Torsin subfamily. TOR2A contain two peptides Salusin-alpha and Salusin-beta. Salusins -alpha and -beta may be endocrine and/or paracrine factors able to increase intracellular calcium concentrations and induce cell mitogenesis. Salusins may also be potent hypotensive peptides.

实验方案

Product Specific Protocols
WB protocol for TOR2A antibody 19511-1-APDownload protocol
IHC protocol for TOR2A antibody 19511-1-APDownload protocol
IF protocol for TOR2A antibody 19511-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Death Dis

Salusin-β contributes to oxidative stress and inflammation in diabetic cardiomyopathy.

Authors - Ming-Xia Zhao
View Article
humanWB

Cell Death Dis

REV1 promotes lung tumorigenesis by activating the Rad18/SERTAD2 axis.

Authors - Yunshang Chen
View Article