验证数据展示
经过测试的应用
| Positive WB detected in | COLO 320 cells, HeLa cells, HEK-293T cells |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy TOR1AIP1 Ready-To-Use IHC Kit. TOR1AIP1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21459-1-AP targets TOR1AIP1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IP, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag14823 Product name: Recombinant human TOR1AIP1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-218 aa of BC023247 Sequence: MKTRRTTRLQQQHSEQPPLQPSPVTTRRGLRDSHSSEEDEASSQTDLSQTISKKTVRSIQEAPVSEDLVIRLRRPPLRYPRYEATSVQQKVNFSEEGETEEDDQDSSHSSVTTVKARSRDSDESGDKTTRSSSQYIESFWQSSQSQNFTAHDKQRSVLSSGYQKTPQEWAPQTARIRTRMQNDSILKSELGNQSPSTSSRQVTGQPQNASFVKRNRWW 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | torsin A interacting protein 1 |
| 别名 | LAP1B, LAP1, Torsin-1A-interacting protein 1, TOR1AIP 1, LGMD2Y |
| 计算分子量 | 462aa,52 kDa; 583aa,66 kDa |
| 观测分子量 | 50-55 kDa |
| GenBank蛋白编号 | BC023247 |
| 基因名称 | TOR1AIP1 |
| Gene ID (NCBI) | 26092 |
| RRID | AB_2878861 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5JTV8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for TOR1AIP1 antibody 21459-1-AP | Download protocol |
| WB protocol for TOR1AIP1 antibody 21459-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biochem Biophys Res Commun Analysis of the GFP-labelled β-dystroglycan interactome in HEK-293 transfected cells reveals novel intracellular networks | ||
Nat Cell Biol LAP1 supports nuclear adaptability during constrained melanoma cell migration and invasion |
