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验证数据展示

  • WB analysis using 84049-1-RR (same clone as 84049-1-PBS)

    WB analysis using 84049-1-RR (same clone as 84049-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84049-1-RR (TOP2B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84049-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 84049-1-RR (same clone as 84049-1-PBS)

    WB analysis of HepG2 using 84049-1-RR (same clone as 84049-1-PBS)

    WB result of TOP2B antibody (84049-1-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOP2B transfected HepG2 cells. This data was developed using the same antibody clone with 84049-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84049-1-RR (same clone as 84049-1-PBS)

    IF Staining of HeLa using 84049-1-RR (same clone as 84049-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TOP2B antibody (84049-1-RR, Clone: 240949F10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84049-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

TOP2B Recombinant monoclonal antibody, PBS Only

TOP2B Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84049-1-PBS

产品说明书

CloneNo. 240949F10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

240949F10, DNA topoisomerase 2-beta, EC:5.6.2.2, top2beta, TOPIIB

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84049-1-PBS targets TOP2B in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 topoisomerase (DNA) II beta 180kDa
别名 240949F10, DNA topoisomerase 2-beta, EC:5.6.2.2, top2beta, TOPIIB
计算分子量 183 kDa
观测分子量180-190 kDa
GenBank蛋白编号NM_001068
基因名称 TOP2B
Gene ID (NCBI) 7155
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ02880
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TOP2B belongs to the type II topoisomerase family. TOP2B control topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2B makes double-strand breaks. It is indirectly ivolved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.

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