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验证数据展示

  • IHC staining of human tonsillitis using 82738-10-RR

    IHC staining of human tonsillitis using 82738-10-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82738-10-RR (TOP2A-Specific antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 82738-10-RR

    IHC staining of human tonsillitis using 82738-10-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82738-10-RR (TOP2A-Specific antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • SEC-HPLC of 82738-10-RR

    SEC-HPLC of 82738-10-RR

    The purity of Human TOP2A-Specific Recombinant monoclonal antibody (Cat. No. 82738-10-RR) was greater than 95% as determined by SEC-HPLC.

  • IF Staining of U2OS using 82738-10-RR

    IF Staining of U2OS using 82738-10-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using TOP2A-Specific antibody (82738-10-RR, Clone: 253104A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

TOP2A-Specific Recombinant monoclonal antibody

TOP2A-Specific Uni-rAb® Recombinant Antibody for IHC, IF/ICC, ELISA
Cat No. 82738-10-RR

产品说明书

CloneNo. 253104A1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, ELISA

别名

TOP2A, DNA topoisomerase 2 alpha, DNA topoisomerase 2-alpha, EC:5.6.2.2, TOP II

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82738-10-RR targets TOP2A-Specific in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 topoisomerase (DNA) II alpha 170kDa
别名 TOP2A, DNA topoisomerase 2 alpha, DNA topoisomerase 2-alpha, EC:5.6.2.2, TOP II
计算分子量 174 kDa
GenBank蛋白编号NM_001067
基因名称 TOP2A
Gene ID (NCBI) 7153
RRIDAB_3743420
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP11388
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TOP2A, also named as TOP2, belongs to the type II topoisomerase family. Topoisomerase II makes double-strand breaks. TOP2 control the topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2A may play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation. DNA topoisomerase IIa (170 kDa, TOP2a/170) is essential in proliferating cells by resolving DNA topological entanglements during chromosome condensation, replication, and segregation.

实验方案

Product Specific Protocols
IF protocol for TOP2A-Specific antibody 82738-10-RRDownload protocol
IHC protocol for TOP2A-Specific antibody 82738-10-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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