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验证数据展示

  • IHC staining of human breast cancer using 16689-1-AP

    IHC staining of human breast cancer using 16689-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16689-1-AP (TOMM6 antibody) at dilution of 1:50 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 16689-1-AP

    IHC staining of human breast cancer using 16689-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16689-1-AP (TOMM6 antibody) at dilution of 1:50 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of human skeletal muscle using 16689-1-AP

    WB analysis of human skeletal muscle using 16689-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16689-1-AP (TOMM6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

TOMM6 Polyclonal antibody

TOMM6 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16689-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

OBTP, TOM6, TOMM6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman skeletal muscle tissue, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16689-1-AP targets TOMM6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TOMM6 fusion protein Ag10037 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 translocase of outer mitochondrial membrane 6 homolog (yeast)
别名 OBTP, TOM6, TOMM6
计算分子量 74 aa, 8 kDa
观测分子量 8 kDa
GenBank蛋白编号BC018624
基因名称 TOMM6
Gene ID (NCBI) 100188893
RRIDAB_2240919
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96B49
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TOMM6, also named as OBTP and TOM6, is a part of the preprotein translocase complex of the outer mitochondrial membrane (TOM complex).

实验方案

Product Specific Protocols
WB protocol for TOMM6 antibody 16689-1-APDownload protocol
IHC protocol for TOMM6 antibody 16689-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols