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验证数据展示

  • IHC staining of human breast cancer using 16689-1-AP

    IHC staining of human breast cancer using 16689-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16689-1-AP (TOMM6 antibody) at dilution of 1:50 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 16689-1-AP

    IHC staining of human breast cancer using 16689-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16689-1-AP (TOMM6 antibody) at dilution of 1:50 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of human skeletal muscle using 16689-1-AP

    WB analysis of human skeletal muscle using 16689-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16689-1-AP (TOMM6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

TOMM6 Polyclonal antibody

TOMM6 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16689-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

OBTP, TOM6, TOMM6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman skeletal muscle tissue, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16689-1-AP targets TOMM6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag10037

Product name: Recombinant human TOMM6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-74 aa of BC018624

Sequence: MASSTVPVSAAGSANETPEIPDNVGDWLRGVYRFATDRNDFRRNLILNLGLFAAGVWLARNLSDIDLMAPQPGV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 translocase of outer mitochondrial membrane 6 homolog (yeast)
别名 OBTP, TOM6, TOMM6
计算分子量 74 aa, 8 kDa
观测分子量 8 kDa
GenBank蛋白编号BC018624
基因名称 TOMM6
Gene ID (NCBI) 100188893
RRIDAB_2240919
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96B49
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TOMM6, also named as OBTP and TOM6, is a part of the preprotein translocase complex of the outer mitochondrial membrane (TOM complex).

实验方案

Product Specific Protocols
WB protocol for TOMM6 antibody 16689-1-APDownload protocol
IHC protocol for TOMM6 antibody 16689-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols