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验证数据展示

  • IHC staining of mouse embryo using 20053-1-AP

    IHC staining of mouse embryo using 20053-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 20053-1-AP (TNS3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse embryo using 20053-1-AP

    IHC staining of mouse embryo using 20053-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 20053-1-AP (TNS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of mouse kidney using 20053-1-AP

    WB analysis of mouse kidney using 20053-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20053-1-AP (TNS3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

TNS3 Polyclonal antibody

TNS3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20053-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

H_NH049I23.2, TEM6, TENS1, tensin 3, TNS3, TPP, Tumor endothelial marker 6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse kidney tissue
Positive IHC detected inmouse embryo tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

20053-1-AP targets TNS3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tensin 3
别名 H_NH049I23.2, TEM6, TENS1, tensin 3, TNS3, TPP, Tumor endothelial marker 6
计算分子量 155 kDa
观测分子量 129 kDa
GenBank蛋白编号NM_022748
基因名称 TNS3
Gene ID (NCBI) 64759
RRIDAB_10667405
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ68CZ2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TNS3, also named as TEM6, TENS1 and TPP, may play a role in actin remodeling. It is involved in the dissociation of the integrin-tensin-actin complex. EGF activates TNS4 and down-regulates TNS3 which results in capping the tail of ITGB1. TNS3 seems to be involved in mammary cell migration. It may be involved in cell migration and bone development. The antibody recognizes all the isoforms of TNS3.

实验方案

Product Specific Protocols
WB protocol for TNS3 antibody 20053-1-APDownload protocol
IHC protocol for TNS3 antibody 20053-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Biol Toxicol

EIF4A3-mediated oncogenic circRNA hsa_circ_0001165 advances esophageal squamous cell carcinoma progression through the miR-381-3p/TNS3 pathway

Authors - Xun Zhang
View Article