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TNS3 Polyclonal antibody

TNS3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 20053-1-AP

Print datasheet

Synonyms

H_NH049I23.2, TEM6, TENS1, tensin 3, TNS3, TPP, Tumor endothelial marker 6

验证数据展示

  • IHC staining of mouse embryo using 20053-1-AP

    IHC staining of mouse embryo using 20053-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 20053-1-AP (TNS3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse embryo using 20053-1-AP

    IHC staining of mouse embryo using 20053-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 20053-1-AP (TNS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of mouse kidney using 20053-1-AP

    WB analysis of mouse kidney using 20053-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20053-1-AP (TNS3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse kidney tissue
Positive IHC detected inmouse embryo tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20053-1-AP targets TNS3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tensin 3
Synonyms H_NH049I23.2, TEM6, TENS1, tensin 3, TNS3, TPP, Tumor endothelial marker 6
Calculated Molecular Weight 155 kDa
Observed Molecular Weight 129 kDa
GenBank Accession NumberNM_022748
Gene Symbol TNS3
Gene ID (NCBI) 64759
RRIDAB_10667405
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ68CZ2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TNS3, also named as TEM6, TENS1 and TPP, may play a role in actin remodeling. It is involved in the dissociation of the integrin-tensin-actin complex. EGF activates TNS4 and down-regulates TNS3 which results in capping the tail of ITGB1. TNS3 seems to be involved in mammary cell migration. It may be involved in cell migration and bone development. The antibody recognizes all the isoforms of TNS3.

实验方案

Product Specific Protocols
WB protocol for TNS3 antibody 20053-1-APDownload protocol
IHC protocol for TNS3 antibody 20053-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols