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验证数据展示

  • WB analysis of fetal human brain using 17831-1-AP

    WB analysis of fetal human brain using 17831-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 17831-1-AP (TNPO2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 17831-1-AP

    IF Staining of MCF-7 using 17831-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using TNPO2 antibody (17831-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Featured Product
  • KD/KO验证

TNPO2 Polyclonal antibody

TNPO2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 17831-1-AP

产品说明书

引用文献(5)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA and More (2)

别名

TNPO 2, KPNB2B, Karyopherin beta-2b, IPO3, Importin 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected infetal human brain tissue, HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17831-1-AP targets TNPO2 in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB, IF, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原

CatNo: Ag12229

Product name: Recombinant human TNPO2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-350 aa of BC072420

Sequence: MDWQPDEQGLQQVLQLLKDSQSPNTATQRIVQDKLKQLNQFPDFNNYLIFVLTRLKSEDEPTRSLSGLILKNNVKAHYQSFPPPVADFIKQECLNNIGDASSLIRATIGILITTIASKGELQMWPELLPQLCNLLNSEDYNTCEGAFGALQKICEDSSELLDSDALNRPLNIMIPKFLQFFKHCSPKIRSHAIACVNQFIMDRAQALMDNIDTFIEHLFALAVDDDPEVRKNVCRALVMLLEVRIDRLIPHMHSIIQYMLQRTQDHDENVALEACEFWLTLAEQPICKEVLASHLVQLIPILVNGMKYSEIDIILLKGDVEEDEAVPDSEQDIKPRFHKSRTVTLPHEAE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transportin 2
别名 TNPO 2, KPNB2B, Karyopherin beta-2b, IPO3, Importin 3
计算分子量 887 aa, 100 kDa
观测分子量 90-100 kDa
GenBank蛋白编号BC072420
基因名称 TNPO2
Gene ID (NCBI) 30000
RRIDAB_10598481
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14787
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TNPO2 antibody 17831-1-APDownload protocol
IF protocol for TNPO2 antibody 17831-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Cell Rep

Hypertonic Stress Causes Cytoplasmic Translocation of Neuronal, but Not Astrocytic, FUS due to Impaired Transportin Function.

Authors - Eva-Maria Hock
View Article
humanWB,IF

Sci Rep

Transportin-2 plays a critical role in nucleocytoplasmic shuttling of oestrogen receptor-α.

Authors - Tetsuji Moriyama
  • KD Validated
View Article
mouseWB

eLife

Kap-β2/Transportin mediates β-catenin nuclear transport in Wnt signaling

Authors - Woong Y Hwang
View Article
humanWB,CoIP

Int J Biol Macromol

Membrane-bound transcription factor LRRC4 inhibits glioblastoma cell motility

Authors - Yang Liu
View Article
humanWB

J Biol Chem

The nuclear transportation of CHRONO regulates the circadian rhythm

Authors - Qin Zhou
View Article