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  • IF Staining of mouse testis using 17178-1-AP (same clone as 17178-1-PBS)

    IF Staining of mouse testis using 17178-1-AP (same clone as 17178-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 17178-1-AP (TNP1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17178-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse testis using 17178-1-AP (same clone as 17178-1-PBS)

    WB analysis of mouse testis using 17178-1-AP (same clone as 17178-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 17178-1-AP (TNP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17178-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse testis using 17178-1-AP (same clone as 17178-1-PBS)

    IF Staining of mouse testis using 17178-1-AP (same clone as 17178-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse testis tissue using 17178-1-AP (TNP1 antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17178-1-PBS in a different storage buffer formulation.

TNP1 Polyclonal antibody, PBS Only

TNP1 Polyclonal Antibody for WB, IF-P, IF-Fro, Indirect ELISA
Cat No. 17178-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF-P, IF-Fro, Indirect ELISA

别名

STP-1, TP-1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

17178-1-PBS targets TNP1 in WB, IF-P, IF-Fro, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF-P, IF-Fro, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag10890

Product name: Recombinant human TNP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-55 aa of BC029516

Sequence: MSTSRKLKSHGMRRSKSRSPHKGVKRGGSKRKYRKGNLKSRKRGDDANRNYRSHL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transition protein 1 (during histone to protamine replacement)
别名 STP-1, TP-1
计算分子量 55 aa, 7 kDa
观测分子量 6-10 kDa
GenBank蛋白编号BC029516
基因名称 TNP1
Gene ID (NCBI) 7141
RRIDAB_2206757
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP09430
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Transition nuclear proteins (TNP1 and TNP2) are the major nuclear proteins that replace somatic histones during spermatogenesis. TNPs are required for normal chromatin condensation and functional sperm development, spermatogenesis was found to be compromised in both Tnp1 and Tnp2 null mice. TNP1, or TP1, localized in nucleus, is a spermatid-specific product of the haploid genome which replaces histone and is itself replaced in the mature sperm by the protamines. Recently, TNP-1 was used as a germ cell marker in condensing spermatids.

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