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验证数据展示

  • WB analysis of rat skeletal muscle using 19729-1-AP

    WB analysis of rat skeletal muscle using 19729-1-AP

    rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19729-1-AP (TNNT3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 19729-1-AP

    IHC staining of mouse skeletal muscle using 19729-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 19729-1-AP (TNNT3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 19729-1-AP

    IHC staining of mouse skeletal muscle using 19729-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 19729-1-AP (TNNT3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TNNT3 Polyclonal antibody

TNNT3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 19729-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Troponin T-FS, Troponin T3 skeletal fast, Fast skeletal muscle troponin T, Beta-TnTF

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inrat skeletal muscle tissue, mouse skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19729-1-AP targets TNNT3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 troponin T type 3 (skeletal, fast)
别名 Troponin T-FS, Troponin T3 skeletal fast, Fast skeletal muscle troponin T, Beta-TnTF
计算分子量 32 kDa
观测分子量 35-40 kDa
GenBank蛋白编号NM_001042780
基因名称 TNNT3
Gene ID (NCBI) 7140
RRIDAB_2878601
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDP45378
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TNNT3 antibody 19729-1-APDownload protocol
IHC protocol for TNNT3 antibody 19729-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Phytomedicine

9-PAHSA ameliorates microvascular damage during cardiac ischaemia/reperfusion injury by promoting LKB1/AMPK/ULK1-mediated autophagy-dependent STING degradation

Authors - Wenhu Liu
View Article