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TNNT3 Polyclonal antibody

TNNT3 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 19729-1-AP

Print datasheet

Synonyms

Fast skeletal muscle troponin T, TNNT3, Troponin T3 skeletal fast, Troponin T-FS

验证数据展示

  • WB analysis of rat skeletal muscle using 19729-1-AP

    WB analysis of rat skeletal muscle using 19729-1-AP

    rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19729-1-AP (TNNT3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skeletal muscle using 19729-1-AP

    IHC staining of human skeletal muscle using 19729-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 19729-1-AP (TNNT3 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skeletal muscle using 19729-1-AP

    IHC staining of human skeletal muscle using 19729-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 19729-1-AP (TNNT3 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inrat skeletal muscle tissue
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19729-1-AP targets TNNT3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name troponin T type 3 (skeletal, fast)
Synonyms Fast skeletal muscle troponin T, TNNT3, Troponin T3 skeletal fast, Troponin T-FS
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberNM_001042780
Gene Symbol TNNT3
Gene ID (NCBI) 7140
RRIDAB_2878601
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDP45378
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TNNT3 antibody 19729-1-APDownload protocol
IHC protocol for TNNT3 antibody 19729-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols