验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, rat heart tissue, HEK-293 cells |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | mouse heart tissue, human heart tissue, mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
| Positive FC (Intra) detected in | A-673 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:300-1:1200 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21652-1-AP targets Cardiac Troponin I in WB, IHC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, ELISA |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag16365 Product name: Recombinant human TNNI3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-110 aa of BC096165 Sequence: MADGSSDAAREPRPAPAPIRRRSSNYRAYATEPHAKKKSKISASRKLQLKTLLLQIAKQELEREAEERRGEKGRALSTRCQPLELAGLGFAELQDLCRQLHARVDKVDEE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | troponin I type 3 (cardiac) |
| 别名 | cTnI, TNNI3 |
| 计算分子量 | 210 aa, 24 kDa |
| 观测分子量 | 24-26 kDa |
| GenBank蛋白编号 | BC096165 |
| 基因名称 | Cardiac Troponin I |
| Gene ID (NCBI) | 7137 |
| RRID | AB_2878898 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P19429 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Troponin I (TnI) is the inhibitory subunit of troponin; the thin filament regulatory complex that confers calcium sensitivity to striated muscle actomyosin ATPase activity. Cardiac Troponin I (cTnI) is encoded by the TNNI3 gene located on chromosome 19 in humans.
What is the molecular weight of cTnI?
The molecular weight of cTnI is 24 kDa.
What is the tissue specificity of cTnI?
cTnI is produced exclusively by cardiac muscle.
What are the post-translational modifications of cTnI?
cTnI at Ser-23 and Ser-24 under adrenergic stimulation enhances the relaxation of cardiac muscle.
What is the role of cTnI in disease?
cTnI is considered to be one of the most specific and sensitive markers of myocardial damage such as acute myocardial infarction (AMI), as detection of cTnI in serum is widely accepted as a diagnostic tool (PMID: 15452154). Defects in TNNI3 lead to cardiomyopathy familial hypertrophic type 7 (CMH7); an inherited heart disorder characterized by ventricular hypertrophy (PMID: 8205619). Defects in TNNI3 can also cause cardiomyopathy familial restrictive type I (RCM1) (PMID: 20617149) and cardiomyopathy dilated type 2A (CMD2A) (PMID: 12531876). These disorders are characterized by the impaired filling of ventricles or ventricular dilation respectively.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for Cardiac Troponin I antibody 21652-1-AP | Download protocol |
| IF protocol for Cardiac Troponin I antibody 21652-1-AP | Download protocol |
| IHC protocol for Cardiac Troponin I antibody 21652-1-AP | Download protocol |
| IP protocol for Cardiac Troponin I antibody 21652-1-AP | Download protocol |
| WB protocol for Cardiac Troponin I antibody 21652-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
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|---|---|---|
Signal Transduct Target Ther Thiamine-modified metabolic reprogramming of human pluripotent stem cell-derived cardiomyocyte under space microgravity | ||
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Theranostics Localized injection of miRNA-21-enriched extracellular vesicles effectively restores cardiac function after myocardial infarction. | ||
Theranostics miR-17-3p Contributes to Exercise-Induced Cardiac Growth and Protects against Myocardial Ischemia-Reperfusion Injury. | ||
Adv Sci (Weinh) An Alternating Current Electroosmotic Flow-Based Ultrasensitive Electrochemiluminescence Microfluidic System for Ultrafast Monitoring, Detection of Proteins/miRNAs in Unprocessed Samples | ||
Adv Sci (Weinh) Injectable BMSC-Based Extracellular Matrix-Mimicking Microtissue for Myocardial Infarction Repair. |
