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验证数据展示

  • WB analysis using 22253-1-AP

    WB analysis using 22253-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22253-1-AP (TNNI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skeletal muscle using 22253-1-AP

    IHC staining of human skeletal muscle using 22253-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22253-1-AP (TNNI1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skeletal muscle using 22253-1-AP

    IHC staining of human skeletal muscle using 22253-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22253-1-AP (TNNI1 antibody) at dilution of 1:50 (under 40x lens).

TNNI1 Polyclonal antibody

TNNI1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22253-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

SSTNI, TNN1, TNNI1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle
Positive IHC detected inhuman skeletal muscle tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22253-1-AP targets TNNI1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TNNI1 fusion protein Ag17749 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 troponin I type 1 (skeletal, slow)
别名 SSTNI, TNN1, TNNI1
计算分子量 187 aa, 22 kDa
观测分子量 25 kDa
GenBank蛋白编号BC012600
基因名称 TNNI1
Gene ID (NCBI) 7135
RRIDAB_11182276
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP19237
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TNNI1 antibody 22253-1-APDownload protocol
IHC protocol for TNNI1 antibody 22253-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols