验证数据展示
经过测试的应用
| Positive WB detected in | mouse skeletal muscle tissue, human skeletal muscle tissue, rat skeletal muscle tissues |
| Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse skeletal muscle tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 6 publications below |
| IF | See 2 publications below |
产品信息
16102-1-AP targets TNNI1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag9001 Product name: Recombinant human TNNI1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-187 aa of BC012600 Sequence: MPEVERKPKITASRKLLLKSLMLAKAKECWEQEHEEREAEKVRYLAERIPTLQTRGLSLSALQDLCRELHAKVEVVDEERYDIEAKCLHNTREIKDLKLKVMDLRGKFKRPPLRRVRVSADAMLRALLGSKHKVSMDLRANLKSVKKEDTEKERPVEVGDWRKNVEAMSGMEGRKKMFDAAKSPTSQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | troponin I type 1 (skeletal, slow) |
| 别名 | SSTNI, TNN1, Troponin I, slow-twitch isoform |
| 计算分子量 | 187 aa, 22 kDa |
| 观测分子量 | 25 kDa |
| GenBank蛋白编号 | BC012600 |
| 基因名称 | TNNI1 |
| Gene ID (NCBI) | 7135 |
| RRID | AB_2206103 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P19237 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for TNNI1 antibody 16102-1-AP | Download protocol |
| IHC protocol for TNNI1 antibody 16102-1-AP | Download protocol |
| WB protocol for TNNI1 antibody 16102-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Basic Res Cardiol Alkaline nucleoplasm facilitates contractile gene expression in the mammalian heart. | ||
iScience Engineering of thick human functional myocardium via static stretching and electrical stimulation. | ||
Sci Rep A missense mutation in the RSRSP stretch of Rbm20 causes dilated cardiomyopathy and atrial fibrillation in mice. | ||
Front Physiol Lipidomic Analyses Reveal Specific Alterations of Phosphatidylcholine in Dystrophic Mdx Muscle. | ||
Cell Death Discov Long noncoding RNA LINC00284 facilitates cell proliferation in papillary thyroid cancer via impairing miR-3127-5p targeted E2F7 suppression. | ||
Front Med Distinct mononuclear diploid cardiac subpopulation with minimal cell-cell communications persists in embryonic and adult mammalian heart |
