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验证数据展示

  • WB analysis of mouse skeletal muscle using 13504-1-AP (same clone as 13504-1-PBS)

    WB analysis of mouse skeletal muscle using 13504-1-AP (same clone as 13504-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13504-1-AP (TNNC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13504-1-PBS in a different storage buffer formulation.

  • IHC staining of human skeletal muscle using 13504-1-AP (same clone as 13504-1-PBS)

    IHC staining of human skeletal muscle using 13504-1-AP (same clone as 13504-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 13504-1-AP (TNNC1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 13504-1-PBS in a different storage buffer formulation.

  • IHC staining of human skeletal muscle using 13504-1-AP (same clone as 13504-1-PBS)

    IHC staining of human skeletal muscle using 13504-1-AP (same clone as 13504-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 13504-1-AP (TNNC1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 13504-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse heart using 13504-1-AP (same clone as 13504-1-PBS)

    IP experiment of mouse heart using 13504-1-AP (same clone as 13504-1-PBS)

    IP result of anti-TNNC1 (IP:13504-1-AP, 4ug; Detection:13504-1-AP 1:600) with mouse heart tissue lysate 4400ug. This data was developed using the same antibody clone with 13504-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 13504-1-AP (same clone as 13504-1-PBS)

    IF Staining of U2OS using 13504-1-AP (same clone as 13504-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using TNNC1 antibody (13504-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13504-1-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 13504-1-AP (same clone as 13504-1-PBS)

    FC experiment of U2OS using 13504-1-AP (same clone as 13504-1-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.4 ug TNNC1 Polyclonal antibody (13504-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 13504-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TNNC1 Polyclonal antibody, PBS Only

TNNC1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 13504-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

别名

TN C, TNC, TN-C, TNNC, Troponin C

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

13504-1-PBS targets TNNC1 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4339

Product name: Recombinant human TNNC1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-161 aa of BC030244

Sequence: MDDIYKAAVEQLTEEQKNEFKAAFDIFVLGAEDGCISTKELGKVMRMLGQNPTPEELQEMIDEVDEDGSGTVDFDEFLVMMVRCMKDDSKGKSEEELSDLFRMFDKNADGYIDLDELKIMLQATGETITEDDIEELMKDGDKNNDGRIDYDEFLEFMKGVE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 troponin C type 1 (slow)
别名 TN C, TNC, TN-C, TNNC, Troponin C
计算分子量 161 aa, 18 kDa
观测分子量 18 kDa
GenBank蛋白编号BC030244
基因名称 TNNC1
Gene ID (NCBI) 7134
RRIDAB_2256271
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP63316
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Troponin is a central regulatory protein of striated muscle contraction, and together with tropomyosin, is located on the actin filament. Troponin consists of 3 subunits: TnI, which is the inhibitor of actomyosin ATPase; TnT, which contains the binding site for tropomyosin; and TnC, the protein encoded by this gene. TnC plays an important role in excitation-contraction coupling and is the primary target of compounds such as levosimendan that augment Ca2+ responsiveness of the thin filament.

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