验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, L02 cells, MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55323-1-AP targets TNKS1BP1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tankyrase 1 binding protein 1, 182kDa |
| 别名 | TAB182 |
| 计算分子量 | 182 kDa |
| 观测分子量 | 182 kDa |
| GenBank蛋白编号 | NM_033396 |
| 基因名称 | TNKS1BP1 |
| Gene ID (NCBI) | 85456 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9C0C2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TNKS1BP1, also known as TAB182, is a member of the poly(ADP-ribose) polymerase (PARP) superfamily. It is an anchor polymerase 1 (Tankyrase1) binding protein with a molecular weight of 182 kDa and is widely distributed throughout the cell, including the cell membrane, cytoplasm, and nucleus. TNKS1BP1 functions in both telomere maintenance and DNA damage response. TNKS1BP1 can compete with TRF1 to bind to the multiple binding sites of tankyrase1, potentially interfering with the transport of tankyrase1 from cytoplasm to nucleus and its localization in telomeres, which affects the stability of telomeres (PMID: 28058814, PMID: 39019859, PMID: 39113018).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TNKS1BP1 antibody 55323-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
