- Featured Product
- KD/KO Validated
TNKS Polyclonal antibody
TNKS Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, Jurkat cells, MCF-7 cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | mouse testis tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy TNKS Ready-To-Use IHC Kit. TNKS primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18030-1-AP targets TNKS in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | TNKS fusion protein Ag12597 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase |
| Synonyms | PARP 5a, PARP5A, PARPL, TANK1, Tankyrase 1, Tankyrase I, TIN1, TINF1, TNKS, TNKS 1, TNKS1 |
| Calculated Molecular Weight | 1327 aa, 142 kDa |
| Observed Molecular Weight | 150 kDa |
| GenBank Accession Number | BC098394 |
| Gene Symbol | TNKS |
| Gene ID (NCBI) | 8658 |
| RRID | AB_2303564 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95271 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TNKS antibody 18030-1-AP | Download protocol |
| IHC protocol for TNKS antibody 18030-1-AP | Download protocol |
| IF protocol for TNKS antibody 18030-1-AP | Download protocol |
| IP protocol for TNKS antibody 18030-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Biol Chem The dePARylase NUDT16 promotes radiation resistance of cancer cells by blocking SETD3 for degradation via reversing its ADP-ribosylation | ||
Oncol Lett Dihydroartemisinin suppresses proliferation, migration, the Wnt/β-catenin pathway and EMT via TNKS in gastric cancer. | ||
Cell Death Dis circ5615 functions as a ceRNA to promote colorectal cancer progression by upregulating TNKS.
| ||
Chem Biol Interact Identification and evaluation of a novel PARP1 inhibitor for the treatment of triple-negative breast cancer |
