验证数据展示
经过测试的应用
| Positive WB detected in | SKOV-3 cells, SW 1990 cells, LoVo cells, Caki-1 cells, Caki-2 cells, SCaBER cells, Raji cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66450-1-Ig targets 4-1BBL/TNFSF9 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag12768 Product name: Recombinant human TNFSF9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 53-254 aa of BC104805 Sequence: WAVSGARASPGSAASPRLREGPELSPDDPAGLLDLRQGMFAQLVAQNVLLIDGPLSWYSDPGLAGVSLTGGLSYKEDTKELVVAKAGVYYVFFQLELRRVVAGEGSGSVSLALHLQPLRSAAGAAALALTVDLPPASSEARNSAFGFQGRLLHLSAGQRLGVHLHTEARARHAWQLTQGATVLGLFRVTPEIPAGLPSPRSE 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | tumor necrosis factor (ligand) superfamily, member 9 |
| 别名 | TNFSF9, 1B4B1, 4 1BB L, 4 1BB ligand, 4 1BBL |
| 计算分子量 | 254 aa, 27 kDa |
| 观测分子量 | 27 kDa |
| GenBank蛋白编号 | BC104805 |
| 基因名称 | TNFSF9 |
| Gene ID (NCBI) | 8744 |
| RRID | AB_2881819 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P41273 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TNFSF9, also named as 4-1BBL, belongs to the tumor necrosis factor family. It is a cytokine that binds to TNFRSF9. TNFSF9 induces the proliferation of activated peripheral blood T-cells. It may have a role in activation-induced cell death (AICD) and may play a role in cognate interactions between T-cells and B-cells/macrophages.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for 4-1BBL/TNFSF9 antibody 66450-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Aging (Albany NY) TNFSF9 promotes metastasis of pancreatic cancer through Wnt/Snail signaling and M2 polarization of macrophages.
| ||
Int Immunopharmacol TNFSF9 promotes metastasis of pancreatic cancer by regulating M2 polarization of macrophages through Src/FAK/p-Akt/IL-1β signaling
| ||
BMC Cancer Clinical significance of nonerythrocytic spectrin Beta 1 (SPTBN1) in human kidney renal clear cell carcinoma and uveal melanoma: a study based on Pan-Cancer Analysis | ||
Hepatol Commun HBV infection effects prognosis and activates the immune response in intrahepatic cholangiocarcinoma | ||