验证数据展示
经过测试的应用
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17852-1-AP targets TNFSF8 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IHC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag12303 Product name: Recombinant human TNFSF8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 61-234 aa of BC093630 Sequence: VVQRTDSIPNSPDNVPLKGGNCSEDLLCILKRAPFKKSWAYLQVAKHLNKTKLSWNKDGILHGVRYQDGNLVIQFPGLYFIICQLQFLVQCPNNSVDLKLELLINKHIKKQALVTVCESGMQTKHVYQNLSQFLLDYLQVNTTISVNVDTFQYIDTSTFPLENVLSIFLYSNSD 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tumor necrosis factor (ligand) superfamily, member 8 |
| 别名 | CD153, CD30 L, CD30 ligand, CD30L, CD30LG, TNFSF8 |
| 计算分子量 | 234 aa, 26 kDa |
| 观测分子量 | 35-40 kDa |
| GenBank蛋白编号 | BC093630 |
| 基因名称 | TNFSF8 |
| Gene ID (NCBI) | 944 |
| RRID | AB_2878452 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P32971 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TNFSF8, also named as CD30LG and CD153, is a member of the tumor necrosis factor (TNF) receptor superfamily, is a surface antigen used as a clinical marker for Hodgkin lymphoma and related hematologic malignancies. It induces proliferation of T-cells. CD30L enhanced the proliferation of CD3-activated T cells, but induced differential responses, including cell death, in several CD30-positive lymphoma-derived cell lines. For Glycosylation, the MW in WB detection is about 35-40 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for TNFSF8 antibody 17852-1-AP | Download protocol |
| WB protocol for TNFSF8 antibody 17852-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
