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验证数据展示

  • IHC staining of human spleen using 14194-1-AP

    IHC staining of human spleen using 14194-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 14194-1-AP (TNFSF14 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TNFSF14/LIGHT antibody included
  • IHC staining of human spleen using 14194-1-AP

    IHC staining of human spleen using 14194-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 14194-1-AP (TNFSF14 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TNFSF14/LIGHT antibody included

LIGHT Polyclonal antibody

LIGHT Polyclonal Antibody for IHC, ELISA
Cat No. 14194-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, ELISA

别名

CD258, HVEM L, HVEML, LIGHT, LTg, TNFSF14, TR2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TNFSF14/LIGHT Ready-To-Use IHC Kit. TNFSF14/LIGHT primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14194-1-AP targets LIGHT in IHC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag5380

Product name: Recombinant human LIGHT protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-177 aa of BC018058

Sequence: MEESVVRPSVFVVDGQTDIPFTRLGRSHRRQSCSVARVGLGLLLLLMGAGLAVQGWFLLQLHWRLGEMVTRLPDGPAGSWEQLIQERRSHEVNPAAHLTGANSSLTGSGGPLLWETQLGVAFLRGLSYHDGALVVTKAGYYYIYSKVQLGGVGCPLGLASTITHGLYKRTPRYPEEL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tumor necrosis factor (ligand) superfamily, member 14
别名 CD258, HVEM L, HVEML, LIGHT, LTg, TNFSF14, TR2
计算分子量 26 kDa
GenBank蛋白编号BC018058
基因名称 LIGHT
Gene ID (NCBI) 8740
RRIDAB_2256332
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43557
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LIGHT, also known as TNFSF14, HVEML, or CD258, is a type II transmembrane protein that belongs to the TNF superfamily (PMID: 9462508). It is predominantly expressed in lymphoid tissues and produced by activated T cells and immature dendritic cells (DCs) (PMID: 9462508; 10754304). Through its two primary functional receptors HVEM (TNFRSF14) and lymphotoxin β receptor (LTβR), LIGHT signaling is involved in development and maintenance of lymphoid tissues, as well as innate and adaptive immune responses (PMID: 24575096; 26720335). In addition to the membrane form, LIGHT can also exist as a soluble form generated by cleavage of the extracellular portion of the membrane form.

实验方案

Product Specific Protocols
IHC protocol for LIGHT antibody 14194-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols