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LIGHT Polyclonal antibody

LIGHT Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat No : 14194-1-AP

Print datasheet

Synonyms

CD258, HVEM L, HVEML, LIGHT, LTg, TNFSF14, TR2

验证数据展示

  • IHC staining of human spleen using 14194-1-AP

    IHC staining of human spleen using 14194-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 14194-1-AP (TNFSF14 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TNFSF14/LIGHT antibody included
  • IHC staining of human spleen using 14194-1-AP

    IHC staining of human spleen using 14194-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 14194-1-AP (TNFSF14 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TNFSF14/LIGHT antibody included

经过测试的应用

Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TNFSF14/LIGHT Ready-To-Use IHC Kit. TNFSF14/LIGHT primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14194-1-AP targets LIGHT in IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, ELISA
Tested Reactivity human
Immunogen LIGHT fusion protein Ag5380 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tumor necrosis factor (ligand) superfamily, member 14
Synonyms CD258, HVEM L, HVEML, LIGHT, LTg, TNFSF14, TR2
Calculated Molecular Weight 26 kDa
GenBank Accession NumberBC018058
Gene Symbol TNFSF14
Gene ID (NCBI) 8740
RRIDAB_2256332
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43557
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LIGHT, also known as TNFSF14, HVEML, or CD258, is a type II transmembrane protein that belongs to the TNF superfamily (PMID: 9462508). It is predominantly expressed in lymphoid tissues and produced by activated T cells and immature dendritic cells (DCs) (PMID: 9462508; 10754304). Through its two primary functional receptors HVEM (TNFRSF14) and lymphotoxin β receptor (LTβR), LIGHT signaling is involved in development and maintenance of lymphoid tissues, as well as innate and adaptive immune responses (PMID: 24575096; 26720335). In addition to the membrane form, LIGHT can also exist as a soluble form generated by cleavage of the extracellular portion of the membrane form.

实验方案

Product Specific Protocols
IHC protocol for LIGHT antibody 14194-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols