验证数据展示
经过测试的应用
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22582-1-AP targets CD268/BAFF-R in IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag18202 Product name: Recombinant human TNFRSF13C protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-184 aa of BC105123 Sequence: MRRGPRSLRGRDAPAPTPCVPAECFDLLVRHCVACGLLRTPRPKPAGASSPAPRTALQPQESVGAGAGEAALPLPGLLFGAPALLGLALVLALVLVGLVSWRRRQRRLRGASSAEAPDGDKDAPEPLDKVIILSPGISDATAPAWPPPGEDPGTTPPGHSVPVPATELGSTELVTTKTAGPEQQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tumor necrosis factor receptor superfamily, member 13C |
| 别名 | TNFRSF13C, BLyS receptor 3, B-cell-activating factor receptor, BAFF-R, BAFFR |
| 计算分子量 | 184 aa, 19 kDa |
| GenBank蛋白编号 | BC105123 |
| 基因名称 | TNFRSF13C |
| Gene ID (NCBI) | 115650 |
| RRID | AB_2879126 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity purified |
| UNIPROT ID | Q96RJ3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
B-cell-activating factor receptor (BAFF-R, also known as CD268 and TNFRSF13C) is a member of the tumor necrosis factor receptor (TNFR) superfamily. It is expressed in most malignant B cells, including those in primary central nervous system lymphoma (PCNSL), and is involved in the survival and proliferation of these cells (PMID: 32528875). The interaction of BAFF with BAFF-R promotes NF-κB activation, which plays a crucial role in B-cell maturation, survival, and costimulation of T-cell activation and proliferation (PMID: 30349534). Additionally, BAFF-R is associated with chronic kidney disease (CKD) by activating NF-κB signaling in renal tubular epithelial cells (PMID: 38791453).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for CD268/BAFF-R antibody 22582-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
