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TNFRSF13C Polyclonal antibody

TNFRSF13C Polyclonal Antibody for FC, IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, IHC,ELISA

Conjugate

Unconjugated

Cat No : 22582-1-AP

Print datasheet

Synonyms

BAFF R, BAFF receptor, BAFFR, BLyS receptor 3, BR3, CD268, TNFRSF13C

验证数据展示

  • IHC staining of human tonsillitis using 22582-1-AP

    IHC staining of human tonsillitis using 22582-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22582-1-AP (TNFRSF13C Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TNFRSF13C antibody included
  • IHC staining of human tonsillitis using 22582-1-AP

    IHC staining of human tonsillitis using 22582-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22582-1-AP (TNFRSF13C Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TNFRSF13C antibody included
  • FC experiment of Raji using 22582-1-AP

    FC experiment of Raji using 22582-1-AP

    1X10^6 Raji cells were stained with 0.2ug TNFRSF13C antibody (22582-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

经过测试的应用

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inRaji cells
Planning an IHC experiment? We recommend our IHCeasy TNFRSF13C Ready-To-Use IHC Kit. TNFRSF13C primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 22582-1-AP is TNFRSF13C Fusion Protein expressed in E. coli.

Tested Applications FC, IHC,ELISA
Tested Reactivity human
Immunogen TNFRSF13C fusion protein Ag18202 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tumor necrosis factor receptor superfamily, member 13C
Synonyms BAFF R, BAFF receptor, BAFFR, BLyS receptor 3, BR3, CD268, TNFRSF13C
Calculated Molecular Weight 184 aa, 19 kDa
GenBank Accession NumberBC105123
Gene Symbol TNFRSF13C
Gene ID (NCBI) 115650
RRIDAB_2879126
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ96RJ3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for TNFRSF13C antibody 22582-1-APDownload protocol
FC protocol for TNFRSF13C antibody 22582-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols