TNFR2 / TNFRSF1B Polyclonal antibody
TNFR2 / TNFRSF1B Polyclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | THP-1 cells, U-937 cells |
Positive IHC detected in | human tonsillitis tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | THP-1 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:5000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
28746-1-AP targets TNFR2 / TNFRSF1B in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.
Tested Applications | IF/ICC, IHC, WB, ELISA |
Tested Reactivity | Human |
Immunogen | TNFR2 / TNFRSF1B fusion protein Ag30490 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | tumor necrosis factor receptor superfamily, member 1B |
Synonyms | CD120b, Etanercept, p75, p75TNFR, p80 TNF alpha receptor, TBP 2, TBPII, TNF R II, TNF R2, TNF R75, TNF RII, TNFBR, TNFR II, TNFR1B, TNFR2, TNFR80, TNFRSF1B |
Calculated Molecular Weight | 48 kDa |
Observed Molecular Weight | 75 kDa, 65 kDa |
GenBank Accession Number | BC052977 |
Gene Symbol | TNFRSF1B |
Gene ID (NCBI) | 7133 |
RRID | AB_2881205 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P20333 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for TNFR2 / TNFRSF1B antibody 28746-1-AP | Download protocol |
IHC protocol for TNFR2 / TNFRSF1B antibody 28746-1-AP | Download protocol |
IF protocol for TNFR2 / TNFRSF1B antibody 28746-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |