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验证数据展示

  • WB analysis using 85759-4-RR (same clone as 85759-4-PBS)

    WB analysis using 85759-4-RR (same clone as 85759-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85759-4-RR (TIPE2/TNFAIP8L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85759-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 85759-4-RR (same clone as 85759-4-PBS)

    IHC staining of mouse spleen using 85759-4-RR (same clone as 85759-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85759-4-RR (TNFAIP8L2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85759-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85759-4-RR

    Affinity and Kinetic Characterization of 85759-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85759-4-RR against Human TNFAIP8L2 were performed. The affinity constant is below 1 pM.

TIPE2/TNFAIP8L2 Recombinant monoclonal antibody, PBS Only

TIPE2/TNFAIP8L2 Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 85759-4-PBS

产品说明书

CloneNo. 250047D9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

TIPE2, Inflammation factor protein 20, TNF alpha-induced protein 8-like protein 2, TNFAIP8 like protein 2, TNFAIP8L2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85759-4-PBS targets TIPE2/TNFAIP8L2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7964

Product name: Recombinant human TNFAIP8L2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-184 aa of BC063014

Sequence: MESFSSKSLALQAEKKLLSKMAGRSVAHLFIDETSSEVLDELYRVSKEYTHSRPQAQRVIKDLIKVAIKVAVLHRNGSFGPSELALATRFRQKLRQGAMTALSFGEVDFTFEAAVLAGLLTECRDVLLELVEHHLTPKSHGRIRHVFDHFSDPGLLTALYGPDFTQHLGKICDGLRKLLDEGKL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tumor necrosis factor, alpha-induced protein 8-like 2
别名 TIPE2, Inflammation factor protein 20, TNF alpha-induced protein 8-like protein 2, TNFAIP8 like protein 2, TNFAIP8L2
计算分子量 184 aa, 21 kDa
观测分子量18 kDa
GenBank蛋白编号BC063014
基因名称 TIPE2
Gene ID (NCBI) 79626
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ6P589
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TIPE2 (TNF-α-inducible protein 8-like 2, or TNFAIP8L2), a negative immune regulator, is essential for maintaining immune homeostasis. TIPE2 shares considerable sequence homology with members of the tumor necrosis factor-α-inducible protein 8 (TNFAIP8) family, which are thought to regulate cellular and immune homeostasis. TIPE2 acts as a negative regulator of Toll-like receptor and T-cell receptor function and is preferentially expressed in lymphoid tissues. TIPE2 plays an essential role in a signal transduction pathway that links the inflammatory immune response to specific conditions after cerebral ischemia

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