验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
23456-1-AP targets TNFAIP3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig |
| 免疫原 | TNFAIP3 fusion protein Ag18150 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tumor necrosis factor, alpha-induced protein 3 |
| 别名 | OTUD7C, OTU domain-containing protein 7C, EC:2.3.2.-, A20p50, A20p37 |
| 计算分子量 | 790 aa, 90 kDa |
| 观测分子量 | 80-90 kDa, 37-40 kDa |
| GenBank蛋白编号 | BC114480 |
| 基因名称 | TNFAIP3 |
| Gene ID (NCBI) | 7128 |
| RRID | AB_2879284 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P21580 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TNFAIP3, also named A20, is a cytoplasmic zinc finger protein that inhibits nuclear factor kappa-B (NFKB) activity and tumor necrosis factor (TNF)-mediated programmed cell death. A20 is a ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities, and involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors (TLRs) through terminating NF-kappa-B activity. Upon T- or B-cell receptor engagement human (h) A20 is cleaved by MALT1 after arginine 439, yielding an N-terminal fragment and a C-terminal one (~37-40 kDa). This antibody, which is a rabbit polyclonal antibody raised against 350 aa of C-terminus of human TNFAIP3, can recognize full-length A20 (~90 kDa) and cleaved form (~37-40 kDa).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TNFAIP3 antibody 23456-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Total Environ USP15 participates in DBP-induced testicular oxidative stress injury through regulating the Keap1/Nrf2 signaling pathway. | ||
Am J Cancer Res ZY-444 inhibits the growth and metastasis of prostate cancer by targeting TNFAIP3 through TNF signaling pathway | ||
Biomedicines The Cholinergic Anti-Inflammatory Pathway Attenuates the Development of Atherosclerosis in Apoe-/- Mice through Modulating Macrophage Functions. | ||
Biomedicines Sertoli Cells Express Accommodation, Survival, and Immunoregulatory Factors When Exposed to Normal Human Serum | ||
Int Immunopharmacol TNFAIP3-upregulated RIP3 exacerbates acute pancreatitis via activating NLRP3 inflammasome. | ||
Mol Cell Biol LncRNA HOXA-AS3 integrates NF-κB signaling to regulate endothelium inflammation. |
