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验证数据展示

  • WB analysis of HeLa using 15320-1-AP

    WB analysis of HeLa using 15320-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15320-1-AP (TNFAIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 15320-1-AP

    IHC staining of human placenta using 15320-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 15320-1-AP (TNFAIP1 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with TNFAIP1 antibody included
  • IHC staining of human placenta using 15320-1-AP

    IHC staining of human placenta using 15320-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 15320-1-AP (TNFAIP1 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with TNFAIP1 antibody included
  • IF Staining of HeLa using 15320-1-AP

    IF Staining of HeLa using 15320-1-AP

    Immunofluorescent analysis of HeLa cells using 15320-1-AP (TNFAIP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

TNFAIP1 Polyclonal antibody

TNFAIP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15320-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Tumor necrosis factor, alpha-induced protein 1, endothelial, EDP1, BTB/POZ domain-containing protein TNFAIP1, BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, L02 cells
Positive IHC detected inhuman placenta tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TNFAIP1 Ready-To-Use IHC Kit. TNFAIP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:300-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15320-1-AP targets TNFAIP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原

CatNo: Ag0220

Product name: Recombinant human TNFAIP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 71-239 aa of BC001949

Sequence: ILIDRCGKHFGTILNYLRDDTITLPQNRQEIKELMAEAKYYLIQGLVNMCQSALQDKKDSYQPVCNIPIITSLKEEERLIESSTKPVVKLLYNRSNNKYSYTSNSDDHLLKNIELFDKLSLRFNGRVLFIKDVIGDEICCWSFYGQGRKLAEVCCTSIVYATEKKQTKV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tumor necrosis factor, alpha-induced protein 1 (endothelial)
别名 Tumor necrosis factor, alpha-induced protein 1, endothelial, EDP1, BTB/POZ domain-containing protein TNFAIP1, BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2
计算分子量 36 kDa
观测分子量 36 kDa
GenBank蛋白编号BC001949
基因名称 TNFAIP1
Gene ID (NCBI) 7126
RRIDAB_10644288
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13829
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TNFAIP1 was identified as a human gene activated transcriptionally by tumour necrosis factor alpha in endothelial cells. It is encoded by a 3.5-kilobase transcript and its expression is found to be developmentally regulated in a tissue-specific manner. The open reading frame predicts a 316-amino acid sequence rich in charged residues, particularly at the carboxyl terminus.

实验方案

Product Specific Protocols
IF protocol for TNFAIP1 antibody 15320-1-APDownload protocol
IHC protocol for TNFAIP1 antibody 15320-1-APDownload protocol
WB protocol for TNFAIP1 antibody 15320-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Commun Signal

Neuronal-specific TNFAIP1 ablation attenuates postoperative cognitive dysfunction via targeting SNAP25 for K48-linked ubiquitination

Authors - Wei Wang
View Article
mouseWB

Neurotox Res

TNFAIP1 Mediates Formaldehyde-Induced Neurotoxicity by Inhibiting the Akt/CREB Pathway in N2a Cells.

Authors - Junzhi Yi
  • KD Validated
View Article
humanWB

Oncol Lett

MicroRNA-373 is upregulated and targets TNFAIP1 in human gastric cancer, contributing to tumorigenesis.

Authors - Zhang Xiaoting X
  • KD Validated
View Article
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