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验证数据展示

  • FC experiment of human PBMCs using 83721-6-PBS

    FC experiment of human PBMCs using 83721-6-PBS

    1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug TNF-alpha Recombinant monoclonal antibody, PBS Only (Detector) (83721-6-PBS, Clone: 240668A4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Cells were co-stained with 0.25 ug CoraLite® Plus 647 Anti-Human CD4 (RPA-T4) (CL647-65143, Clone: RPA-T4). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

  • FC experiment of human PBMCs using 83721-6-RR (same clone as 83721-6-PBS)

    FC experiment of human PBMCs using 83721-6-RR (same clone as 83721-6-PBS)

    1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug TNF-Alpha Recombinant Antibody (83721-6-RR, Clone:240668A4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), and 0.25 ug APC Anti-Human CD4 (RPA-T4) (APC-65143, Clone: RPA-T4), and 0.25 ug Control Antibody. Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 83721-6-PBS in a different storage buffer formulation.

  • ELISA experiment of Recombinant protein using 83721-6-PBS

    ELISA experiment of Recombinant protein using 83721-6-PBS

    0.7 ug/mL Human TNFα (Eg0816) was coated to the plate, and incubated with serial concentration of biotin- labeled 83721-6-PBS. EC50 was 0.0099 nM.

  • ELISA experiment of human PBMCs using 83721-6-RR (same clone as 83721-6-PBS)

    ELISA experiment of human PBMCs using 83721-6-RR (same clone as 83721-6-PBS)

    1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug TNF-alpha Recombinant monoclonal antibody, PBS Only (Detector) (83721-6-PBS, Clone:240668A4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), or 0.25 ug APC Anti-Human CD4 (RPA-T4) (APC-65143, Clone: RPA-T4). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 83721-6-PBS in a different storage buffer formulation.

  • ELISA experiment of peptide using 83721-6-RR (same clone as 83721-6-PBS)

    ELISA experiment of peptide using 83721-6-RR (same clone as 83721-6-PBS)

    ELISA was performed using antibody 83721-6-RR at concentrations ranging from 0.02 to 2000 ng/mL to detect the TNF-alpha peptide. HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a 1:10,000 dilution. This data was developed using the same antibody clone with 83721-6-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP00702-4

    Sandwich ELISA sample test of MP00702-4

    Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days. The mean TNF-alpha concentration was determined to be 1.3 pg/mL in unstimulated PBMC supernatant,1236.3 pg/mL in PHA stimulated PBMC supernatant.

  • Sandwich ELISA sample test of MP00702-4

    Sandwich ELISA sample test of MP00702-4

    Serum of seven individual healthy human donors was measured. The human TNF-alpha concentration of detected samples was determined to be 3.25 pg/mL with a range of ND-9.1 pg/mL.

  • Sandwich ELISA standard curve of MP00702-4

    Sandwich ELISA standard curve of MP00702-4

    Sandwich ELISA standard curve of MP00702-4, TNF-alpha Recombinant Matched Antibody Pair - PBS only. 83721-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0816. 83721-6-PBS was HRP conjugated as the detection antibody. Range: 3.9-250 pg/mL.

  • Affinity and Kinetic Characterization of 83721-6-PBS

    Affinity and Kinetic Characterization of 83721-6-PBS

    Biolayer interferometry (BLl) kinetic assay of 83721-6-PBS against Human TNF-alpha was performed. The affinity constant is 47.9 pM.

  • Affinity of 83721-6-RR

    Affinity of 83721-6-RR

    Biolayer interferometry (BLI) kinetic assays of 83721-6-RR against Human TNF-alpha were performed. The affinity constant is below 1 pM.

TNF-alpha Recombinant monoclonal antibody, PBS Only (Detector)

TNF-alpha Uni-rAb® Recombinant Antibody for FC (Intra), ELISA, Sandwich ELISA, Indirect ELISA
Cat No. 83721-6-PBS

产品说明书

CloneNo. 240668A4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra), ELISA, Sandwich ELISA, Indirect ELISA

别名

TNF, TNF alpha, TNF-a, C-domain 1, C-domain 2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inPMA, Ionomycin and Brefeldin A treated human PBMCs
Positive ELISA detected inRecombinant protein
Planning an IHC experiment? We recommend our IHCeasy TNF Ready-To-Use IHC Kit. TNF primary antibody included.

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Enzyme-linked Immunosorbent Assay (ELISA)ELISA : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83721-6-PBS targets TNF-alpha as part of a matched antibody pair:

MP00702-4: 83721-5-PBS capture and 83721-6-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 FC (Intra), ELISA, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg0816

Product name: Recombinant Human TNF-alpha protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 77-233 aa of NM_000594.4

Sequence: VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tumor necrosis factor (TNF superfamily, member 2)
别名 TNF, TNF alpha, TNF-a, C-domain 1, C-domain 2
计算分子量26 kDa
GenBank蛋白编号NM_000594.4
基因名称 TNF-alpha
Gene ID (NCBI) 7124
ENSEMBL Gene IDENSG00000232810
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP01375
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, ins resistance, and cancer.

实验方案

Product Specific Protocols
FC protocol for TNF-alpha antibody 83721-6-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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