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验证数据展示

  • WB analysis using 27789-1-AP

    WB analysis using 27789-1-AP

    U-87 MG cells were subjected to SDS PAGE followed by western blot with 27789-1-AP (TNC antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 27789-1-AP

    IHC staining of human liver cancer using 27789-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27789-1-AP (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TNC/Tenascin-C antibody included
  • IHC staining of human liver cancer using 27789-1-AP

    IHC staining of human liver cancer using 27789-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27789-1-AP (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TNC/Tenascin-C antibody included

TNC/Tenascin-C Polyclonal antibody

TNC/Tenascin-C Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27789-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IHC, ELISA

别名

Cytotactin, GMEM, GP 150 225, Hexabrachion, HXB, JI, Myotendinous antigen, Neuronectin, Tenascin, tenascin C, TN, TN C, TNC

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inU-87 MG cells
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TNC/Tenascin-C Ready-To-Use IHC Kit. TNC/Tenascin-C primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27789-1-AP targets TNC/Tenascin-C in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human
免疫原 TNC/Tenascin-C fusion protein Ag27122 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tenascin C
别名 Cytotactin, GMEM, GP 150 225, Hexabrachion, HXB, JI, Myotendinous antigen, Neuronectin, Tenascin, tenascin C, TN, TN C, TNC
计算分子量 2201 aa, 241 kDa
GenBank蛋白编号BC151843
基因名称 Tenascin C
Gene ID (NCBI) 3371
RRIDAB_2918131
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP24821
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tenascin-C (TNC) is a large hexameric extracellular matrix glycoprotein of the tenascin family (PMID: 21818551). It is a multimodular protein containing multiple epidermal growth factor (EGF)-like repeats and fibronectin type III (FN III) domains (PMID: 1719530). Tenascin-C is highly expressed during embryonic development, particularly in the developing central nervous system, around motile cells and at epithelial-mesenchymal interaction sites (PMID: 25738825). In adult tissues, the expression and the distribution of TNC are typically limited under normal physiological conditions. It is upregulated during injury, inflammation, regeneration, and cancer (PMID: 7694605; 25120494). Tenascin-C is a diverse protein that can produce different functions including regulating cell adhesion, migration and proliferation.

实验方案

Product Specific Protocols
WB protocol for TNC/Tenascin-C antibody 27789-1-APDownload protocol
IHC protocol for TNC/Tenascin-C antibody 27789-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols