验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, A375 cells, human heart tissue, L02 cells |
| Positive IHC detected in | human brain tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IF | See 1 publications below |
产品信息
21348-1-AP targets TMX4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag15696 Product name: Recombinant human TMX4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 184-349 aa of BC033787 Sequence: PAWCSYVFFVIATLVFGLFMGLVLVVISECFYVPLPRHLSERSEQNRRSEEAHRAEQLQDAEEEKDDSNEEENKDSLVDDEEEKEDLGDEDEAEEEEEEDNLAAGVDEERSEANDQGPPREDGVTREEVEPEEAEEGISEQPCPADTEVVEDSLRQRKSQHADKGL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | thioredoxin-related transmembrane protein 4 |
| 别名 | UNQ475/PRO938, TXNDC13, Thioredoxin-related transmembrane protein 4, Thioredoxin domain-containing protein 13, PDIA14 |
| 计算分子量 | 349 aa, 39 kDa |
| 观测分子量 | 40-45 kDa |
| GenBank蛋白编号 | BC033787 |
| 基因名称 | TMX4 |
| Gene ID (NCBI) | 56255 |
| RRID | AB_10733353 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H1E5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TMX4(Thioredoxin-related transmembrane protein 4) is also named as KIAA1162, TXNDC13. It is a type I transmembrane protein, with a Trx-like domain facing the ER lumen and may be to enable protein folding in cooperation with these proteins consisting of folding complex in the ER. It could work as a reductase in the environment of the ER(PMID: 20056998). The full length protein has a 23 amino acid signal peptide. This antibody is specific to TMX4.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for TMX4 antibody 21348-1-AP | Download protocol |
| IF protocol for TMX4 antibody 21348-1-AP | Download protocol |
| IHC protocol for TMX4 antibody 21348-1-AP | Download protocol |
| WB protocol for TMX4 antibody 21348-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun TMX4-driven LINC complex disassembly and asymmetric autophagy of the nuclear envelope upon acute ER stress | ||
