验证数据展示
经过测试的应用
| Positive WB detected in | mouse skin tissue |
| Positive IHC detected in | human skin cancer tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17953-1-AP targets TMPRSS3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag12435 Product name: Recombinant human TMPRSS3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 262-453 aa of BC074846 Sequence: LYLPKSWTIQVGLVSLLDNPAPSHLVEKIVYHSKYKPKRLGNDIALMKLAGPLTFNEMIQPVCLPNSEENFPDGKVCWTSGWGATEDGGDASPVLNHAAVPLISNKICNHRDVYGGIISPSMLCAGYLTGGVDSCQGDSGGPLVCQERRLWKLVGATSFGIGCAEVNKPGVYTRVTSFLDWIHEQMERDLKT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | transmembrane protease, serine 3 |
| 别名 | TADG12, Serine protease TADG-12, ECHOS1, EC:3.4.21.-, DFNB8 |
| 计算分子量 | 453 aa, 49 kDa |
| 观测分子量 | 40 kDa |
| GenBank蛋白编号 | BC074846 |
| 基因名称 | TMPRSS3 |
| Gene ID (NCBI) | 64699 |
| RRID | AB_2878470 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P57727 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for TMPRSS3 antibody 17953-1-AP | Download protocol |
| WB protocol for TMPRSS3 antibody 17953-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Gene Med TMPRSS3 regulates cell viability and apoptosis processes of HEI-OC1 cells via regulation on circ-Slc4a2, miR-182, and AKT cascade.
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Mol Ther Methods Clin Dev Selection of viral capsids and promoters affects the efficacy of rescue of Tmprss3-deficient cochlea
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