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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68264-1-Ig (same clone as 68264-1-PBS)

    WB analysis using 68264-1-Ig (same clone as 68264-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68264-1-Ig (TMPRSS2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68264-1-PBS in a different storage buffer formulation.

  • WB analysis using 68264-1-Ig (same clone as 68264-1-PBS)

    WB analysis using 68264-1-Ig (same clone as 68264-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68264-1-Ig (TMPRSS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68264-1-PBS in a different storage buffer formulation.

  • WB analysis of pig kidney using 68264-1-Ig (same clone as 68264-1-PBS)

    WB analysis of pig kidney using 68264-1-Ig (same clone as 68264-1-PBS)

    pig kidney tissue were subjected to SDS PAGE followed by western blot with 68264-1-Ig (TMPRSS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68264-1-PBS in a different storage buffer formulation.

TMPRSS2 Monoclonal antibody, PBS Only

TMPRSS2 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68264-1-PBS

产品说明书

CloneNo. 1H7E9

宿主/亚型

Mouse / IgG1

种属反应性

Human, Pig

应用

WB, Indirect ELISA

别名

FLJ41954, PP9284, PRSS10, Serine protease 10, TMPRSS2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

68264-1-PBS targets TMPRSS2 in WB, Indirect ELISA applications and shows reactivity with Human, Pig samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 Human, Pig
免疫原

CatNo: Ag5824

Product name: Recombinant human TMPRSS2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 108-492 aa of BC051839

Sequence: FMGSKCSNSGIECDSSGTCINPSNWCDGVSHCPGGEDENRCVRLYGPNFILQVYSSQRKSWHPVCQDDWNENYGRAACRDMGYKNNFYSSQGIVDDSGSTSFMKLNTSAGNVDIYKKLYHSDACSSKAVVSLRCIACGVNLNSSRQSRIVGGESALPGAWPWQVSLHVQNVHVCGGSIITPEWIVTAAHCVEKPLNNPWHWTAFAGILRQSFMFYGAGYQVEKVISHPNYDSKTKNNDIALMKLQKPLTFNDLVKPVCLPNPGMMLQPEQLCWISGWGATEEKGKTSEVLNAAKVLLIETQRCNSRYVYDNLITPAMICAGFLQGNVDSCQGDSGGPLVTSKNNIWWLIGDTSWGSGCAKAYRPGVYGNVMVFTDWIYRQMRADG

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 transmembrane protease, serine 2
别名 FLJ41954, PP9284, PRSS10, Serine protease 10, TMPRSS2
计算分子量 54 kDa
观测分子量50 kDa
GenBank蛋白编号BC051839
基因名称 TMPRSS2
Gene ID (NCBI) 7113
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDO15393
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TMPRSS2, also named as PRSS10, is a type II transmembrane serine protease which is highly expressed by the epithelium of the human prostate gland. TMPRSS2 may contribute to prostate tumour metastasis via the activation of PAR-2. TMPRSS2 is a Serine protease that proteolytically cleaves and activates the viral spike glycoproteins which facilitate virus-cell membrane fusions. TMPRSS2 is a host cell factor that is critical for the spread of several clinically relevant viruses, including influenza A viruses and coronaviruses (PMID: 23468491, 30626688). SARS-CoV-2 uses the SARS-CoV receptor ACE2 for entry and the serine protease TMPRSS2 for S protein priming. The initial spike protein priming by TMPRSS2 is essential for the entry and viral spread of SARS-CoV-2 through interaction with the ACE2 receptor (PMID: 32142651, 30626688 ). Camostat mesylate, an inhibitor of TMPRSS2, can block SARS-CoV-2 infection of lung cells (PMID: 32142651). The MW of TMPRSS2 is about 65-70 kDa. It can be cleaved into some chains with MW 54 kDa, 31 kDa and 26 kDa (PMID: 25734995, 20382709, 26018085).