验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, human heart tissue, human kidney tissue, human lung tissue, mouse brain tissue, mouse kidney tissue |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human kidney tissue, human colon tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy TMOD3 Ready-To-Use IHC Kit. TMOD3 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IHC | See 2 publications below |
产品信息
12521-1-AP targets TMOD3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | TMOD3 fusion protein Ag3215 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tropomodulin 3 (ubiquitous) |
| 别名 | Tropomodulin 3, Tropomodulin-3, UTMOD, U-Tmod |
| 计算分子量 | 352 aa, 40 kDa |
| 观测分子量 | 40 kDa |
| GenBank蛋白编号 | BC020542 |
| 基因名称 | TMOD3 |
| Gene ID (NCBI) | 29766 |
| RRID | AB_2205324 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NYL9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Tropomodulin 3(TMOD3) belongs to the tropomodulin family, which controls cytoskeletal dynamics by regulating the end-capping of actin filaments. TMOD3 bridges the gap between structural integrity and signaling cascades. TMOD3 regulates cytoskeletal architecture and is implicated in cell adhesion, migration, and mechanotransduction.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TMOD3 antibody 12521-1-AP | Download protocol |
| IHC protocol for TMOD3 antibody 12521-1-AP | Download protocol |
| IP protocol for TMOD3 antibody 12521-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Carcinogenesis MiR-145 modulates the radiosensitivity of non-small cell lung cancer cells by suppression of TMOD3. | ||
Comb Chem High Throughput Screen Clinicopathological Characteristics, Prognosis, and Correlated Tumor Cell Function of Tropomodulin-3 in Pancreatic Adenocarcinoma | ||
Drug Resist Updat TMOD3 accelerated resistance to immunotherapy in KRAS-mutated pancreatic cancer through promoting autophagy-dependent degradation of ASCL4 |
