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验证数据展示

  • WB analysis using 68039-1-Ig

    WB analysis using 68039-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68039-1-Ig (TMOD2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human gliomas using 68039-1-Ig

    IHC staining of human gliomas using 68039-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 68039-1-Ig (TMOD2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TMOD2 Monoclonal antibody

TMOD2 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 68039-1-Ig

产品说明书

CloneNo. 1E7D1

宿主/亚型

Mouse / IgG1

种属反应性

Human, pig, rabbit, chicken

应用

WB, IHC, ELISA

别名

N TMOD, Neuronal tropomodulin, NTMOD, TMOD2, Tropomodulin 2, tropomodulin 2 (neuronal)

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inpig brain tissue, rabbit brain, chicken brain, pig cerebellum, rabbit cerebellum, chicken cerebellum
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68039-1-Ig targets TMOD2 in WB, IHC, ELISA applications and shows reactivity with Human, pig, rabbit, chicken samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, pig, rabbit, chicken
免疫原

CatNo: Ag7262

Product name: Recombinant human TMOD2 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-351 aa of BC064961

Sequence: MALPFQKELEKYKNIDEDELLGKLSEEELKQLENVLDDLDPESAMLPAGFRQKDQTQKAATGPFDREHLLMYLEKEALEQKDREDFVPFTGEKKGRVFIPKEKPIETRKEEKVTLDPELEEALASASDTELYDLAAVLGVHNLLNNPKFDEETANNKGGKGPVRNVVKGEKVKPVFEEPPNPTNVEISLQQMKANDPSLQEVNLNNIKNIPIPTLREFAKALETNTHVKKFSLAATRSNDPVAIAFADMLKVNKTLTSLNIESNFITGTGILALVEALKENDTLTEIKIDNQRQQLGTAVEMEIAQMLEENSRILKFGYQFTKQGPRTRVAAAITKNNDLVRKKRVEADRR

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 tropomodulin 2 (neuronal)
别名 N TMOD, Neuronal tropomodulin, NTMOD, TMOD2, Tropomodulin 2, tropomodulin 2 (neuronal)
计算分子量 39 kDa
观测分子量39-40 kDa
GenBank蛋白编号BC064961
基因名称 TMOD2
Gene ID (NCBI) 29767
RRIDAB_2918781
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9NZR1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for TMOD2 antibody 68039-1-IgDownload protocol
WB protocol for TMOD2 antibody 68039-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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