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验证数据展示

  • WB analysis using 84130-6-RR

    WB analysis using 84130-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84130-6-RR (TMIGD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse small intestine using 84130-6-RR

    IF Staining of mouse small intestine using 84130-6-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using TMIGD1 antibody (84130-6-RR, Clone: 250723F6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of rat small intestine using 84130-6-RR

    IF Staining of rat small intestine using 84130-6-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat small intestine tissue using TMIGD1 antibody (84130-6-RR, Clone: 250723F6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TMIGD1 Recombinant monoclonal antibody

TMIGD1 Uni-rAb® Recombinant Antibody for WB, IF-P, ELISA
Cat No. 84130-6-RR

产品说明书

CloneNo. 250723F6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, dog

应用

WB, IF-P, ELISA

别名

TMIGD, Transmembrane and immunoglobulin domain-containing protein 1, UNQ9372/PRO34164

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inDaudi cells, HUVEC cells, Raji cells, MDCK cells
Positive IF-P detected inrat small intestine tissue, mouse small intestine tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84130-6-RR targets TMIGD1 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat, dog samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat, dog
免疫原

CatNo: Ag25691

Product name: Recombinant human TMIGD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 133-207 aa of BC137201

Sequence: VEEGSNVKLVCNVKANPQAQMMWYKNSSLLDLEKSRHQIQQTSESFQLSITKVEKPDNGTYSCIAKSSLKTESLD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transmembrane and immunoglobulin domain containing 1
别名 TMIGD, Transmembrane and immunoglobulin domain-containing protein 1, UNQ9372/PRO34164
计算分子量 262 aa, 29 kDa
观测分子量 45 kDa, 29 kDa
GenBank蛋白编号BC137201
基因名称 TMIGD1
Gene ID (NCBI) 388364
RRIDAB_3743523
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ6UXZ0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMIGD1 (Transmembrane and immunoglobulin domain containing 1) is involved in various cellular processes such as regulation of cell structure, cell-cell adhesion, and cellular movement. It protects cells from oxidative- and nutrient-deprivation-induced cell injury by stabilizing the transepithelial electric resistance and permeability of renal epithelial cells. TMIGD1 has a calculated molecular mass of 29 kDa, and can be detected as 45 kDa due to the N-glycosylation (PMID: 26342724).

实验方案

Product Specific Protocols
IF protocol for TMIGD1 antibody 84130-6-RRDownload protocol
WB protocol for TMIGD1 antibody 84130-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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