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验证数据展示

  • WB analysis of mouse kidney using 27174-1-AP

    WB analysis of mouse kidney using 27174-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 27174-1-AP (TMIGD1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 27174-1-AP

    IHC staining of mouse kidney using 27174-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27174-1-AP (TMIGD1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 27174-1-AP

    IHC staining of mouse kidney using 27174-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27174-1-AP (TMIGD1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 27174-1-AP

    WB analysis using 27174-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27174-1-AP (TMIGD1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of rat small intestine using 27174-1-AP

    IF Staining of rat small intestine using 27174-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat small intestine tissue using TMIGD1 antibody (27174-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse small intestine using 27174-1-AP

    IF Staining of mouse small intestine using 27174-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using TMIGD1 antibody (27174-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TMIGD1 Polyclonal antibody

TMIGD1 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 27174-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, ELISA and More (1)

别名

TMIGD, Transmembrane and immunoglobulin domain-containing protein 1, UNQ9372/PRO34164

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse kidney tissue, Daudi cells, HUVEC cells, Raji cells
Positive IHC detected inmouse kidney tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat small intestine tissue, mouse small intestine tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27174-1-AP targets TMIGD1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 TMIGD1 fusion protein Ag25691 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane and immunoglobulin domain containing 1
别名 TMIGD, Transmembrane and immunoglobulin domain-containing protein 1, UNQ9372/PRO34164
计算分子量 262 aa, 29 kDa
观测分子量 45 kDa, 29 kDa
GenBank蛋白编号BC137201
基因名称 TMIGD1
Gene ID (NCBI) 388364
RRIDAB_2880786
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6UXZ0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMIGD1 (Transmembrane and immunoglobulin domain containing 1) is involved in various cellular processes such as regulation of cell structure, cell-cell adhesion, and cellular movement. It protects cells from oxidative- and nutrient-deprivation-induced cell injury by stabilizing the transepithelial electric resistance and permeability of renal epithelial cells. TMIGD1 has a calculated molecular mass of 29 kDa, and can be detected as 45 kDa due to the N-glycosylation (PMID: 26342724).

实验方案

Product Specific Protocols
WB protocol for TMIGD1 antibody 27174-1-APDownload protocol
IHC protocol for TMIGD1 antibody 27174-1-APDownload protocol
IF protocol for TMIGD1 antibody 27174-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,humanWB,IF

Sci Signal

Intestinal brush border formation requires a TMIGD1-based intermicrovillar adhesion complex

Authors - Christian Hartmann
View Article
humanWB,IHC

Int Immunopharmacol

Macrophage-derived exosomes promote intestinal mucosal barrier dysfunction in inflammatory bowel disease by regulating TMIGD1 via mircroRNA-223

Authors - Xin Chang
View Article