TMF1-Specific Polyclonal antibody

TMF1-Specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 19728-1-AP

Print datasheet

Synonyms

ARA160, TATA element modulatory factor, TMF, TMF1, TMF1-Specific



经过测试的应用

Positive WB detected inBxPC-3 cells, L02 cells, Hela cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman cervical cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy TMF1 Ready-To-Use IHC Kit. TMF1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19728-1-AP targets TMF1-Specific in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF, IHC, IP, WB, ELISA
Cited Applications IF, WB
Tested Reactivity human
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name TATA element modulatory factor 1
Synonyms ARA160, TATA element modulatory factor, TMF, TMF1, TMF1-Specific
Calculated Molecular Weight 123 kDa
Observed Molecular Weight 150-160 kDa
GenBank Accession NumberNM_007114
Gene Symbol TMF1
Gene ID (NCBI) 7110
RRIDAB_10667009
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP82094
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMF1, also named as ARA160, is a potential coactivator of the androgen receptor. TMF1 mediates STAT3 degradation. TMF1 plays critical roles in two RAB6-dependent retrograde transport processes: one from endosomes to the Golgi and the other from the Golgi to the ER. TMF1 binds the HIV-1 TATA element and inhibits transcriptional activation by the TATA-binding protein (TBP). The antibody is specific to TMF1. TMF1 can be detected as 150-160 kDa after posttranslational modification.

实验方案

Product Specific Protocols
WB protocol for TMF1-Specific antibody 19728-1-APDownload protocol
IHC protocol for TMF1-Specific antibody 19728-1-APDownload protocol
IF protocol for TMF1-Specific antibody 19728-1-APDownload protocol
IP protocol for TMF1-Specific antibody 19728-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Mol Cell

An mTORC1-GRASP55 signaling axis controls unconventional secretion to reshape the extracellular proteome upon stress.

Authors - Julian Nüchel
WB

J Biol Chem

Discovery Proteomics Identifies a Molecular Link between the Coatomer Protein Complex I and Androgen Receptor-dependent Transcription.

Authors - Jordy J Hsiao