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验证数据展示

  • WB analysis of rat liver using 24331-1-AP

    WB analysis of rat liver using 24331-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 24331-1-AP (TMEM9B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse colon using 24331-1-AP

    WB analysis of mouse colon using 24331-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 24331-1-AP (TMEM9B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 24331-1-AP

    IHC staining of human colon cancer using 24331-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 24331-1-AP (TMEM9B Antibody) at dilution of 1:50.

  • IHC staining of human colon cancer using 24331-1-AP

    IHC staining of human colon cancer using 24331-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 24331-1-AP (TMEM9B Antibody) at dilution of 1:50.

TMEM9B Polyclonal antibody

TMEM9B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24331-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, rat, mouse

应用

WB, IHC, ELISA

别名

C11orf15, TMEM9 domain family, member B, TMEM9B, Transmembrane protein 9B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inrat liver tissue, mouse colon tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24331-1-AP targets TMEM9B in WB, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, rat, mouse
文献引用反应性mouse
免疫原

CatNo: Ag19383

Product name: Recombinant human TMEM9B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 80-172 aa of BC040124

Sequence: PDVEAYCLRCECKYEERSSVTIKVTIIIYLSILGLLLLYMVYLTLVEPILKRRLFGHAQLIQSDDDIGDHQPFANAHDVLARSRSRANVLNKV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TMEM9 domain family, member B
别名 C11orf15, TMEM9 domain family, member B, TMEM9B, Transmembrane protein 9B
计算分子量 198 aa, 23 kDa
观测分子量 23 kDa, 35 kDa
GenBank蛋白编号BC040124
基因名称 TMEM9B
Gene ID (NCBI) 56674
RRIDAB_2879497
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NQ34
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM9B, also termed as Transmembrane protein 9B encodes a 198 amino-acidprotein that contains an N-terminal signal peptide, a single transmembrane region, three potential N-glycosylation sites, and three conserved cys-rich domains in the N-terminus. There is a dimer form of TMEM9B protein (PMID: 12359240). TMEM9B mRNA is expressed in a wide range of tissues and cells. TMEM9B is a lysosomal transmembrane protein that regulates the activity of inflammatory signaling pathways.

实验方案

Product Specific Protocols
WB protocol for TMEM9B antibody 24331-1-APDownload protocol
IHC protocol for TMEM9B antibody 24331-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse

Nat Commun

Endosomal chloride/proton exchangers need inhibitory TMEM9 β-subunits for regulation and prevention of disease-causing overactivity

Authors - Rosa Planells-Cases
View Article