验证数据展示
经过测试的应用
Positive WB detected in | rat liver tissue, mouse colon tissue |
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
24331-1-AP targets TMEM9B in WB, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.
经测试应用 | WB, IHC, ELISA Application Description |
经测试反应性 | human, rat, mouse |
免疫原 | TMEM9B fusion protein Ag19383 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | TMEM9 domain family, member B |
别名 | C11orf15, TMEM9 domain family, member B, TMEM9B, Transmembrane protein 9B |
计算分子量 | 198 aa, 23 kDa |
观测分子量 | 23 kDa, 35 kDa |
GenBank蛋白编号 | BC040124 |
基因名称 | TMEM9B |
Gene ID (NCBI) | 56674 |
RRID | AB_2879497 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9NQ34 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TMEM9B, also termed as Transmembrane protein 9B encodes a 198 amino-acidprotein that contains an N-terminal signal peptide, a single transmembrane region, three potential N-glycosylation sites, and three conserved cys-rich domains in the N-terminus. There is a dimer form of TMEM9B protein (PMID: 12359240). TMEM9B mRNA is expressed in a wide range of tissues and cells. TMEM9B is a lysosomal transmembrane protein that regulates the activity of inflammatory signaling pathways.
实验方案
Product Specific Protocols | |
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WB protocol for TMEM9B antibody 24331-1-AP | Download protocol |
IHC protocol for TMEM9B antibody 24331-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |