验证数据展示
经过测试的应用
| Positive WB detected in | mouse E12.5 embryos |
| Positive IP detected in | Y79 cells |
| Positive IHC detected in | mouse embryo tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14731-1-AP targets TMEM98 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag6492 Product name: Recombinant human TMEM98 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 27-226 aa of BC000526 Sequence: CRQRYCRPRDLLQRYDSKPIVDLIGAMETQSEPSELELDDVVITNPHIEAILENEDWIEDASGLMSHCIAILKICHTLTEKLVAMTMGSGAKMKTSASVSDIIVVAKRISPRVDDVVKSMYPPLDPKLLDARTTALLLSVSHLVLVTRNACHLTGGLDWIDQSLSAAEEHLEVLREAALASEPDKGLPGPEGFLQEQSAI 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | transmembrane protein 98 |
| 别名 | Protein TADA1, TMEM98, transmembrane protein 98 |
| 计算分子量 | 25 kDa |
| 观测分子量 | 21-25 kDa |
| GenBank蛋白编号 | BC000526 |
| 基因名称 | TMEM98 |
| Gene ID (NCBI) | 26022 |
| RRID | AB_11182589 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y2Y6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TMEM98, also named as TADA1, composed of 226 amino acid residues, is a potential single-pass transmembrane protein. It is expression in both nucleus and cytoplasm. TMEM98 was found to be over-expressed in most of the hepatocellular carcinoma tissue. MEM98 is not only a prognostic marker for patients with hepatocellular carcinoma but also a novel molecular target associated with intrinsic and acquired chemoresistance of hepatocellular carcinoma (PMID: 24608572).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for TMEM98 antibody 14731-1-AP | Download protocol |
| IP protocol for TMEM98 antibody 14731-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
PLoS Genet The nanophthalmos protein TMEM98 inhibits MYRF self-cleavage and is required for eye size specification. | ||
PLoS Genet Variants in myelin regulatory factor (MYRF) cause autosomal dominant and syndromic nanophthalmos in humans and retinal degeneration in mice. | ||
Aging (Albany NY) The microRNA miR-29c-5p inhibits cell proliferation and migration by targeting TMEM98 in head and neck carcinoma. | ||
Invest Ophthalmol Vis Sci Missense Mutations in the Human Nanophthalmos Gene TMEM98 Cause Retinal Defects in the Mouse.
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