验证数据展示
经过测试的应用
| Positive WB detected in | RAW 264.7 cells, MDA-MB-453s cells, PC-3 cells |
| Positive IHC detected in | human breast cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25091-1-AP targets TMEM87A in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag18715 Product name: Recombinant human TMEM87A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 71-160 aa of BC005335 Sequence: GEPCDLSLNITWYLKSADCYNEIYNFKAEEVELYLEKLKEKRGLSGKYQTSSKLFQNCSELFKTQTFSGDFMHRLPLLGEKQEAKENGTN 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | transmembrane protein 87A |
| 别名 | Elkin1, PSEC0094, TM87A, Transmembrane protein 87A |
| 计算分子量 | 555 aa, 63 kDa |
| 观测分子量 | 63-70 kDa |
| GenBank蛋白编号 | BC005335 |
| 基因名称 | TMEM87A |
| Gene ID (NCBI) | 25963 |
| RRID | AB_2879892 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8NBN3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Transmembrane protein 87A (TMEM87A) represents an evolutionarily conserved transmembrane protein. It is predominantly localized to the Golgi complex and involved in retrograde intracellular trafficking (PMID: 26157166). Moreover, TMEM87A has been identified to localize at the plasma membrane of melanoma cells and facilitate mechanoelectrical transduction. Furthermore, it is functionally characterized as an ion channel expressed in sensory neurons, which is responsible for mediating light touch sensory transduction (PMID: 32228863; 38422143).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for TMEM87A antibody 25091-1-AP | Download protocol |
| WB protocol for TMEM87A antibody 25091-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
