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TMEM87A Polyclonal antibody

TMEM87A Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 25091-1-AP

Print datasheet

Synonyms

TMEM87A, transmembrane protein 87A

验证数据展示

  • WB analysis of MDA-MB-453s using 25091-1-AP

    WB analysis of MDA-MB-453s using 25091-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 25091-1-AP (TMEM87A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 25091-1-AP

    IHC staining of human breast cancer using 25091-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25091-1-AP (TMEM87A Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human breast cancer using 25091-1-AP

    IHC staining of human breast cancer using 25091-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25091-1-AP (TMEM87A Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inMDA-MB-453s cells, PC-3 cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25091-1-AP is TMEM87A Fusion Protein expressed in E. coli.

Tested Applications IHC, WB,ELISA
Tested Reactivity human
Immunogen TMEM87A fusion protein Ag18715 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transmembrane protein 87A
Synonyms TMEM87A, transmembrane protein 87A
Calculated Molecular Weight 555 aa, 63 kDa
Observed Molecular Weight 63 kDa
GenBank Accession NumberBC005335
Gene Symbol TMEM87A
Gene ID (NCBI) 25963
RRIDAB_2879892
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NBN3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TMEM87A antibody 25091-1-APDownload protocol
IHC protocol for TMEM87A antibody 25091-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols