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验证数据展示

  • WB analysis using 27708-1-AP

    WB analysis using 27708-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27708-1-AP (TMEM85 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 27708-1-AP

    IHC staining of human brain using 27708-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27708-1-AP (TMEM85 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TMEM85 antibody included
  • IHC staining of human brain using 27708-1-AP

    IHC staining of human brain using 27708-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27708-1-AP (TMEM85 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TMEM85 antibody included
  • IHC staining of human stomach cancer using 27708-1-AP

    IHC staining of human stomach cancer using 27708-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27708-1-AP (TMEM85 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TMEM85 antibody included
  • IHC staining of mouse brain using 27708-1-AP

    IHC staining of mouse brain using 27708-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27708-1-AP (TMEM85 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TMEM85 antibody included
  • IHC staining of rat brain using 27708-1-AP

    IHC staining of rat brain using 27708-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 27708-1-AP (TMEM85 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TMEM85 antibody included
  • IHC staining of rat heart using 27708-1-AP

    IHC staining of rat heart using 27708-1-AP

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 27708-1-AP (TMEM85 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TMEM85 antibody included

TMEM85 Polyclonal antibody

TMEM85 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27708-1-AP

产品说明书

引用文献(4)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Cell proliferation-inducing gene 17 protein, EMC4, ER membrane protein complex subunit 4, HSPC184, PIG17

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, mouse heart tissue, rat heart tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy® TMEM85 Ready-To-Use IHC Kit. TMEM85 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 4 publications below

产品信息

27708-1-AP targets TMEM85 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag26785

Product name: Recombinant human TMEM85 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-70 aa of BC002583

Sequence: MTAQGGLVANRGRRFKWAIELSGPGGGSRGRSDRGSGQGDSLYPVGYLDKQVPDTSVQETDRILVEKRCW

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane protein 85
别名 Cell proliferation-inducing gene 17 protein, EMC4, ER membrane protein complex subunit 4, HSPC184, PIG17
观测分子量 20 kDa
GenBank蛋白编号BC002583
基因名称 TMEM85
Gene ID (NCBI) 51234
RRIDAB_2880950
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5J8M3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for TMEM85 antibody 27708-1-APDownload protocol
WB protocol for TMEM85 antibody 27708-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

WNK1 is an assembly factor for the human ER membrane protein complex.

Authors - Tino Pleiner
View Article
humanWB

J Cell Biol

A selectivity filter in the ER membrane protein complex limits protein misinsertion at the ER

Authors - Tino Pleiner
View Article
humanWB

bioRxiv

Role of a holo-insertase complex in the biogenesis of biophysically diverse ER membrane proteins

Authors - Katharine R Page
View Article
humanWB

Nat Commun

A small molecule VDAC ligand inhibits ERAD and induces selective cancer cell death via disruption of calcium homeostasis.

Authors - Wenjing Yan
View Article
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