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验证数据展示

  • WB analysis using 27708-1-AP

    WB analysis using 27708-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27708-1-AP (TMEM85 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 27708-1-AP

    IHC staining of human brain using 27708-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27708-1-AP (TMEM85 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 27708-1-AP

    IHC staining of human brain using 27708-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27708-1-AP (TMEM85 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TMEM85 Polyclonal antibody

TMEM85 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27708-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse, rat and More (1)

应用

WB, IHC, ELISA

别名

EMC4, ER membrane protein complex subunit 4, PIG17, TMEM85, transmembrane protein 85

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, mouse heart tissue, rat heart tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

27708-1-AP targets TMEM85 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 Human, Mouse, rat
文献引用反应性human
免疫原

CatNo: Ag26785

Product name: Recombinant human TMEM85 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-70 aa of BC002583

Sequence: MTAQGGLVANRGRRFKWAIELSGPGGGSRGRSDRGSGQGDSLYPVGYLDKQVPDTSVQETDRILVEKRCW

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane protein 85
别名 EMC4, ER membrane protein complex subunit 4, PIG17, TMEM85, transmembrane protein 85
观测分子量 20 kDa
GenBank蛋白编号BC002583
基因名称 TMEM85
Gene ID (NCBI) 51234
RRIDAB_2880950
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5J8M3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for TMEM85 antibody 27708-1-APDownload protocol
WB protocol for TMEM85 antibody 27708-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

WNK1 is an assembly factor for the human ER membrane protein complex.

Authors - Tino Pleiner
View Article
humanWB

J Cell Biol

A selectivity filter in the ER membrane protein complex limits protein misinsertion at the ER

Authors - Tino Pleiner
View Article
humanWB

bioRxiv

Role of a holo-insertase complex in the biogenesis of biophysically diverse ER membrane proteins

Authors - Katharine R Page
View Article
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