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TMEM77 Polyclonal antibody

TMEM77 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 23182-1-AP

Print datasheet

Synonyms

DRAM2, PRO180, RP5 1180E21.1, TMEM77, transmembrane protein 77, UNQ154/PRO180, WWFQ154

验证数据展示

  • WB analysis of K-562 using 23182-1-AP

    WB analysis of K-562 using 23182-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 23182-1-AP (TMEM77 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 23182-1-AP

    IHC staining of human ovary tumor using 23182-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 23182-1-AP (TMEM77 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human ovary tumor using 23182-1-AP

    IHC staining of human ovary tumor using 23182-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 23182-1-AP (TMEM77 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inK-562 cells
Positive IHC detected inhuman ovary tumor tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 23182-1-AP is TMEM77 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB,ELISA
Tested Reactivity human
Immunogen TMEM77 fusion protein Ag19711 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transmembrane protein 77
Synonyms DRAM2, PRO180, RP5 1180E21.1, TMEM77, transmembrane protein 77, UNQ154/PRO180, WWFQ154
Calculated Molecular Weight 266 aa, 30 kDa
Observed Molecular Weight 30 kDa, 60 kDa, 70 kDa
GenBank Accession NumberBC047025
Gene Symbol TMEM77
Gene ID (NCBI) 128338
RRIDAB_2879225
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ6UX65
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TMEM77 antibody 23182-1-APDownload protocol
IHC protocol for TMEM77 antibody 23182-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols