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验证数据展示

  • WB analysis of K-562 using 23182-1-AP

    WB analysis of K-562 using 23182-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 23182-1-AP (TMEM77 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 23182-1-AP

    IHC staining of human ovary tumor using 23182-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 23182-1-AP (TMEM77 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human ovary tumor using 23182-1-AP

    IHC staining of human ovary tumor using 23182-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 23182-1-AP (TMEM77 Antibody) at dilution of 1:50 (under 40x lens).

TMEM77 Polyclonal antibody

TMEM77 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 23182-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

DRAM2, PRO180, RP5 1180E21.1, TMEM77, transmembrane protein 77, UNQ154/PRO180, WWFQ154

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells
Positive IHC detected inhuman ovary tumor tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23182-1-AP targets TMEM77 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag19711

Product name: Recombinant human TMEM77 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 170-266 aa of BC047025

Sequence: ALSMLTCSSVLHSGNFGTDLEQKLHWNPEDKGYVLHMITTAAEWSMSFSFFGFFLTYIRDFQKISLRVEANLHGLTLYDTAPCPINNERTRLLSRDI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane protein 77
别名 DRAM2, PRO180, RP5 1180E21.1, TMEM77, transmembrane protein 77, UNQ154/PRO180, WWFQ154
计算分子量 266 aa, 30 kDa
观测分子量 30 kDa, 60 kDa, 70 kDa
GenBank蛋白编号BC047025
基因名称 TMEM77
Gene ID (NCBI) 128338
RRIDAB_2879225
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ6UX65
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TMEM77 antibody 23182-1-APDownload protocol
IHC protocol for TMEM77 antibody 23182-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols