验证数据展示
经过测试的应用
Positive WB detected in | A2780 cells, Jurkat cells |
Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 2 publications below |
WB | See 5 publications below |
产品信息
20388-1-AP targets TMEM70 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human, mouse |
文献引用反应性 | human, mouse |
免疫原 | TMEM70 fusion protein Ag14226 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | transmembrane protein 70 |
别名 | TMEM70, transmembrane protein 70 |
计算分子量 | 260 aa, 29 kDa |
观测分子量 | 18 kDa |
GenBank蛋白编号 | BC002748 |
基因名称 | TMEM70 |
Gene ID (NCBI) | 54968 |
RRID | AB_10694436 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9BUB7 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TMEM70 belongs to the TMEM70 family. It is involved in biogenesis of mitochondrial ATP synthase. Defects in TMEM70 are a cause of mitochondrial encephalocardiomyopathy neonatal due to ATP synthase deficiency (MT-ATPSD). A publication(PMID:21147908) identifies that TMEM70 gene defect as a pan-ethnic disorder and further redefines it as the most common cause of nuclear-origin ATP synthase deficiency.
实验方案
Product Specific Protocols | |
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WB protocol for TMEM70 antibody 20388-1-AP | Download protocol |
IHC protocol for TMEM70 antibody 20388-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Inflammation IRF-1 Regulates Mitochondrial Respiration and Intrinsic Apoptosis Under Metabolic Stress through ATP Synthase Ancillary Factor TMEM70
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Neurogenetics TMEM70: a mutational hot spot in nuclear ATP synthase deficiency with a pivotal role in complex V biogenesis. | ||
JIMD Rep Common and Novel TMEM70 Mutations in a Cohort of Italian Patients with Mitochondrial Encephalocardiomyopathy. | ||
Life Sci Alliance Mitochondrial stress response triggered by defects in protein synthesis quality control.
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