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TMEM66 Polyclonal antibody

TMEM66 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat No : 29599-1-AP

Print datasheet

Synonyms

FLJ22274, FOAP 7, HSPC035, Protein FOAP 7, SARAF, TMEM66, transmembrane protein 66, UNQ1967/PRO4499, XTP3

验证数据展示

  • IHC staining of mouse pancreas using 29599-1-AP

    IHC staining of mouse pancreas using 29599-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 29599-1-AP (TMEM66 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse pancreas using 29599-1-AP

    IHC staining of mouse pancreas using 29599-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 29599-1-AP (TMEM66 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29599-1-AP targets TMEM66 in IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications IHC, ELISA
Tested Reactivity Human, mouse
Immunogen TMEM66 fusion protein Ag29388 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transmembrane protein 66
Synonyms FLJ22274, FOAP 7, HSPC035, Protein FOAP 7, SARAF, TMEM66, transmembrane protein 66, UNQ1967/PRO4499, XTP3
Calculated Molecular Weight 339 aa, 37 kDa
GenBank Accession NumberBC015012
Gene Symbol TMEM66
Gene ID (NCBI) 51669
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96BY9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM66/SOCE-associated regulatory factor (SARAF) is an endoplasmic reticulum (ER)-resident transmembrane protein that promotes SOCE inactivation and prevents Ca2+ overfilling of the cell. TMEM66 plays a key role in shaping cytosolic Ca2+ signals and determining the content of the major intracellular Ca2+ stores, which is probably important in protecting the cell from Ca2+ overfilling (PMID:37007714). TMEM66 is a highly conserved protein in vertebrates. In mammals, TMEM66 is ubiquitously expressed, but has significantly high transcript levels in the immune and neuronal tissues (PMID:25469256). Moreover, TMEM66 is an androgenresponsive marker for prostate cancer and mTOR-dependent cardiac growth (PMID:32173353).

实验方案

Product Specific Protocols
IHC protocol for TMEM66 antibody 29599-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols