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TMEM65 Polyclonal antibody

TMEM65 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 21913-1-AP

Print datasheet

Synonyms

TMEM65, transmembrane protein 65

验证数据展示

  • WB analysis using 21913-1-AP

    WB analysis using 21913-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21913-1-AP (TMEM65 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis using 21913-1-AP

    WB analysis using 21913-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21913-1-AP (TMEM65 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 21913-1-AP

    IHC staining of human colon cancer using 21913-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21913-1-AP (TMEM65 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 21913-1-AP

    IHC staining of human colon cancer using 21913-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21913-1-AP (TMEM65 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of NIH/3T3 using 21913-1-AP

    IF Staining of NIH/3T3 using 21913-1-AP

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using TMEM65 antibody (21913-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • WB analysis of HeLa using 21913-1-AP

    WB analysis of HeLa using 21913-1-AP

    WB result of TMEM65 antibody (21913-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM65 transfected HeLa cells.

经过测试的应用

Positive WB detected inHEK-293 cells, mouse brain tissue, HeLa cells, rat brain tissue, Neuro-2a cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells, Huh-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21913-1-AP targets TMEM65 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen TMEM65 fusion protein Ag15509 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transmembrane protein 65
Synonyms TMEM65, transmembrane protein 65
Calculated Molecular Weight 240 aa, 26 kDa
Observed Molecular Weight20-25 kDa
GenBank Accession NumberBC032396
Gene Symbol TMEM65
Gene ID (NCBI) 157378
RRIDAB_2918075
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6PI78
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TMEM65 antibody 21913-1-APDownload protocol
IHC protocol for TMEM65 antibody 21913-1-APDownload protocol
IF protocol for TMEM65 antibody 21913-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols