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验证数据展示

  • IF Staining of U2OS using 20430-1-AP

    IF Staining of U2OS using 20430-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using TMEM39A antibody (20430-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • IHC staining of human breast cancer using 20430-1-AP

    IHC staining of human breast cancer using 20430-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20430-1-AP (TMEM39A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U-251 using 20430-1-AP

    IF Staining of U-251 using 20430-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using TMEM39A antibody (20430-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • WB analysis using 20430-1-AP

    WB analysis using 20430-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20430-1-AP (TMEM39A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

TMEM39A Polyclonal antibody

TMEM39A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 20430-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

SUSR2, TMEM39A, transmembrane protein 39A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inU-87 MG cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells, U-251 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20430-1-AP targets TMEM39A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 TMEM39A fusion protein Ag14263 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane protein 39A
别名 SUSR2, TMEM39A, transmembrane protein 39A
计算分子量 488 aa, 56 kDa
观测分子量48-56 kDa
GenBank蛋白编号BC021277
基因名称 TMEM39A
Gene ID (NCBI) 55254
RRIDAB_3085605
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NV64
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transmembrane protein 39A (TMEM39A, also called SUSR2) encoding an ER-localized transmembrane protein, is a susceptibility locus for multiple autoimmune diseases (PMID: 31806350).

实验方案

Product Specific Protocols
WB protocol for TMEM39A antibody 20430-1-APDownload protocol
IHC protocol for TMEM39A antibody 20430-1-APDownload protocol
IF protocol for TMEM39A antibody 20430-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols