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验证数据展示

  • WB analysis using 19919-1-AP

    WB analysis using 19919-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19919-1-AP (TMEM38B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 19919-1-AP

    IHC staining of mouse testis using 19919-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19919-1-AP (TMEM38B antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 19919-1-AP

    IHC staining of mouse testis using 19919-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19919-1-AP (TMEM38B antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 19919-1-AP

    IF Staining of HepG2 using 19919-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TMEM38B antibody (19919-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • WB analysis of MCF-7 using 19919-1-AP

    WB analysis of MCF-7 using 19919-1-AP

    WB result of TMEM38B antibody (19919-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM38B transfected MCF-7 cells.

  • Featured Product
  • KD/KO验证

TMEM38B Polyclonal antibody

TMEM38B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 19919-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

C9orf87, D4Ertd89e, TRIC B, TRICB, Trimeric intracellular cation channel type B

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells
Positive IHC detected inmouse testis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19919-1-AP targets TMEM38B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag13835

Product name: Recombinant human TMEM38B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 162-291 aa of BC000049

Sequence: ERLVKGDWKPEGDEWLKMSYPAKVTLLGSVIFTFQHTQHLAISKHNLMFLYTIFIVATKITMMTTQTSTMTFAPFEDTLSWMLFGWQQPFSSCEKKSEAKSPSNGVGSLASKPVDVASDNVKKKHTKKNE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane protein 38B
别名 C9orf87, D4Ertd89e, TRIC B, TRICB, Trimeric intracellular cation channel type B
计算分子量 291 aa, 33 kDa
观测分子量28 kDa
GenBank蛋白编号BC000049
基因名称 TMEM38B
Gene ID (NCBI) 55151
RRIDAB_2878623
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NVV0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TMEM38B encodes endoplasmic reticulum membrane monovalent cation-specific channel which is involved in the release of calcium (Ca2+) from intracellular stores. TMEM38B, also known as TRIC-B, is one of the two trimeric intracellular cation (TRIC) channels. TRIC-B deficiency causes bone disease due to defective Ca2+ release and signaling in the bone cells. (PMID: 34036147, PMID: 39385871)

实验方案

Product Specific Protocols
IF protocol for TMEM38B antibody 19919-1-APDownload protocol
IHC protocol for TMEM38B antibody 19919-1-APDownload protocol
WB protocol for TMEM38B antibody 19919-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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