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验证数据展示

  • WB analysis using 86724-1-RR (same clone as 86724-1-PBS)

    WB analysis using 86724-1-RR (same clone as 86724-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86724-1-RR (TMEM35 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86724-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 86724-1-RR (same clone as 86724-1-PBS)

    IP experiment of mouse brain using 86724-1-RR (same clone as 86724-1-PBS)

    IP result of anti-TMEM35 (IP:86724-1-RR, 4ug; Detection:86724-1-RR 1:5000) with mouse brain tissue lysate 1960 ug. This data was developed using the same antibody clone with 86724-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 86724-1-RR (same clone as 86724-1-PBS)

    IHC staining of mouse brain using 86724-1-RR (same clone as 86724-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86724-1-RR (TMEM35 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86724-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86724-1-RR

    Affinity and Kinetic Characterization of 86724-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86724-1-RR against Human TMEM35 were performed. The affinity constant is 2.31 nM.

TMEM35 Recombinant monoclonal antibody, PBS Only

TMEM35 Uni-rAb® Recombinant Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 86724-1-PBS

产品说明书

CloneNo. 251680G5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IP, Indirect ELISA

别名

NACHO, Novel acetylcholine receptor chaperone, TMEM35A, transmembrane protein 35

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86724-1-PBS targets TMEM35 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag20589

Product name: Recombinant human TMEM35 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 125-167 aa of BC078658

Sequence: LTCRLLIARKPEDRSSEKKPLPGNAEEQPSLYEKAPQGKVKVS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transmembrane protein 35
别名 NACHO, Novel acetylcholine receptor chaperone, TMEM35A, transmembrane protein 35
计算分子量 167 aa, 18 kDa
观测分子量 20 kDa
GenBank蛋白编号BC078658
基因名称 TMEM35
Gene ID (NCBI) 59353
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ53FP2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TMEM35 is a 167-amino acid peptide with a putative NH2 terminal signal peptide, three hydrophobic transmembrane domains, and a nerve growth factor binding domain.TMEM35is 18kDa and has been shown to localize to membrane-bound structures within cells.

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