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验证数据展示

  • WB analysis using 27267-1-AP (same clone as 27267-1-PBS)

    WB analysis using 27267-1-AP (same clone as 27267-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27267-1-AP (TMEM26 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27267-1-PBS in a different storage buffer formulation.

  • WB analysis using 27267-1-AP (same clone as 27267-1-PBS)

    WB analysis using 27267-1-AP (same clone as 27267-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27267-1-AP (TMEM26 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27267-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 27267-1-AP (same clone as 27267-1-PBS)

    IHC staining of human breast cancer using 27267-1-AP (same clone as 27267-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27267-1-AP (TMEM26 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27267-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 27267-1-AP (same clone as 27267-1-PBS)

    IHC staining of human breast cancer using 27267-1-AP (same clone as 27267-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27267-1-AP (TMEM26 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27267-1-PBS in a different storage buffer formulation.

TMEM26 Polyclonal antibody, PBS Only

TMEM26 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 27267-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

transmembrane protein 26

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

27267-1-PBS targets TMEM26 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag24409

Product name: Recombinant human TMEM26 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 84-188 aa of BC042872

Sequence: ELHHETQYCSIQAEGTSQNTSRKEDFNQTLTSNEQTSRADDLIETAKVFVNNLSTVCEKVWTLGLHQTFLLMLIIGRWLLPIGGGITRDQLSQLLLMFVGTAADI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane protein 26
别名 transmembrane protein 26
计算分子量 368 aa, 42 kDa
观测分子量50 kDa
GenBank蛋白编号BC042872
基因名称 TMEM26
Gene ID (NCBI) 219623
RRIDAB_3742226
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6ZUK4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The Transmembrane protein 26 (TMEM26) gene encodes a protein containing multiple transmembrane fragments. Immunostaining indicated elevated TMEM26 expression in ESCC tumors. Various ESCC cell lines showed a high TMEM26 expression, where its plasma membrane localization was confirmed. The RNAi depletion of TMEM26 in TMEM26-high ESCC cells suppressed EMT-related alterations, including invasion, migration, and marker gene expression. Conversely, TMEM26 overexpression in TMEM26-low ESCC cells promoted these EMT-related alterations. Interestingly, TMEM26 depletion or overexpression did not affect cell growth, indicating its specific involvement in the EMT process. The animal study demonstrated the contributive role of TMEM26 in metastatic ESCC. Mechanistically, TMEM26 promoted NF-κB signaling to accelerate EMT in ESCC cells. The plasma membrane presentation and TJ protein assembly were impaired by TMEM26, which was likely to be another mechanism for EMT regulation by TMEM26 in ESCC. Therefore, TMEM26 disrupted TJ formation and promoted NF-κB signaling during the EMT activation in ESCC (PMID: 37611445). TMEM26 shows features for chain, glycosylation, modified residue (large scale data).

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