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验证数据展示

  • WB analysis using 31728-1-AP

    WB analysis using 31728-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31728-1-AP (TMEM208 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse stomach using 31728-1-AP

    IHC staining of mouse stomach using 31728-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 31728-1-AP (TMEM208 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse stomach using 31728-1-AP

    IHC staining of mouse stomach using 31728-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 31728-1-AP (TMEM208 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TMEM208 Polyclonal antibody

TMEM208 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31728-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

HSND2, HSPC171, SND2, Transmembrane protein 208

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected in37°C incubated mouse brain tissue, 37°C incubated rat brain tissue
Positive IHC detected inmouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31728-1-AP targets TMEM208 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag35668

Product name: Recombinant human TMEM208 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 130-173 aa of BC003080

Sequence: PGRALYLLWVNVLGPWFTADSGTPAPEHNEKRQRRQERRQMKRL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transmembrane protein 208
别名 HSND2, HSPC171, SND2, Transmembrane protein 208
计算分子量 173 aa, 20 kDa
观测分子量 20 kDa
GenBank蛋白编号BC003080
基因名称 TMEM208
Gene ID (NCBI) 29100
RRIDAB_3742465
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BTX3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transmembrane protein 208 (TMEM208) can regulate both autophagy and ER stress. When overexpressed, TMEM208 impaired autophagy as characterized by the decrease of the accumulation of LC3-II, decreased degradation of autophagic substrates, and reduced expression of critical effectors and vital molecules of the ER stress and autophagy processes. (PMID: 23691174)

实验方案

Product Specific Protocols
IHC protocol for TMEM208 antibody 31728-1-APDownload protocol
WB protocol for TMEM208 antibody 31728-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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